1997 Fiscal Year Final Research Report Summary
Cell culture study on the mechanism and control of late graft failure occuring in arterial reconstruction procedures.
| Project/Area Number |
08457306
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Ryukyu University |
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Principal Investigator |
KAMADA Yoshihiko Ryukyu University, Faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (10177561)
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| Co-Investigator(Kenkyū-buntansha) |
SAKUDA Hitoshi Ryukyu University, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助手 (80244309)
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| Project Period (FY) |
1996 – 1997
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| Keywords | autovein graft / endothelial cell / smooth muscle cell / intimal hyperplasia / shear stress / collagen / cell culture / vascular surgery |
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| Research Abstract |
The mechanism causing intimal hyperplasia at the anastomotic site in peripheral revascularization procedures, resulting in late graft failure, is obscure at the present time. This intimal hyperplasia occurs mostly in grafts with poor distal outflow, at regions of low shear stress. We studied the effects of shear stress on type IV and type IV collagen production by cultured canine vascular endothelial cells, using disk a rotation device. Semi-quantitative measurements on immunohistochemically processed samples were performed. We found that lower shear stress induced the endothelial cells to produce more type I and type IV collagen. Type IV collagen is a major component of the subendothelial basement membrane. Type I collagen occurs throughout the depth of the hyperplastic intima, and has been shown to promote modulation of cultured arterial smooth muscle cells from a contractile to a synthetic phenotype. We hypothesize that vascular endothelial cells can regulate subendothelial smooth muscle cell activity through control of collagen production in response to shear stress conditions. This mechanism might play an important role in the development intimal hyperplasia seen in the late postoperative period.
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Research Products
(9 results)
