1997 Fiscal Year Final Research Report Summary
Activation of neointima cells on vascular prostheses with permanent antithrombogenic property proceeded from temporally one
| Project/Area Number |
08457310
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
IWAI Yoshihiro Yokohama City University, First Department of Surgery, Assistant Professor, 医学部, 助手 (30275044)
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| Co-Investigator(Kenkyū-buntansha) |
NOISHIKI Yasuharu Yokohama City University, First Department of Surgery, Assistant Professor, 医学部, 講師 (60033263)
MO Makoto Yokohama City University, First Department of Surgery, Assistant Professor, 医学部, 助手 (70264645)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Vascular prosthesis / Neointima / basic fibroblast growth factor / Cytokine reservoir / Endothelial cells / co-culture / Collagen / Negative charge |
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| Research Abstract |
We investigated activities of endothelial cells, and fibroblasts during the neointima formation of a vascular prosthesis. The prosthesis was in a proceeding condition of anthrombogenic property from a temporally and artificial one into a permanent and natural one of endothelial cells. In general, fibroblasts become dominant and endothelial cells is suppressed in a co-culture condition of them in vitro. But in vivo condition, they showed different activities in a wall of an antithrombogenic vascular prosthesis wall.
We beleive that natural and permanent antirthrombogenic property of endothelial cells is the best for vascular prosthesis in order to maintain the graft patency with prevention of thrombus formation on its wall. For the understanding of the cell activity, amount of basic fibroblast growth factor (bFGF) adhered on collagen fibers using RI-raveled bFGF.Collagen fibers with mucopolysaccarides such as chondroitin sulfate and heparan sulfate adsorbed a high amount of bFGF.Negative
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ly charged collagen fibers with succinylation treatment also adsorbed high amount of bFGF in in vitro. Succinylated collagen fibers can prevent platelet adhesion due to its negatively charge, since platelets are also charged negative. As an animal experiment, we implanted a fabric vascular prosthesis coated with succinylated collagen in a descending aorta of dogs. By means of immunohystochemical staining method, we found that the collagen fibers adsorbed a high amount of bFGF after the graft implantation. Numerous fibroblasts with capillary blood vessels migrated into the interstices of Dacron fibers occupied with the succinylated collagen fibers. Both the endothelial cells in the capillary blood vessels and bibroblasts actively created a neointima on the prosthesis wall rapidly. The prosthesis prevented thick thrombus deposition during the process. From the in vivo results, we obtained the following conclusion. Negatively charged collagen fibers prevented thick thrombus deposition. Succinylated collagen fibers worked as a cytokine reservoir on the prosthesis wall. Endothelial cells and fibroblasts did not supress each other in vivo when they were creating neointima formation under a antithrombogenic property on a vascular prosthesis wall. Less
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