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1997 Fiscal Year Final Research Report Summary

Monecular aspect of cholesterol metabolism in gallstone formation

Research Project

Project/Area Number 08457327
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionMIYAZAKI MEDICAL COLLEGE

Principal Investigator

SETOGUCHI Toshiaki  Miyazaki Medical College, Surgery I,Professor, 医学部, 教授 (40041420)

Project Period (FY) 1996 – 1997
Keywordscholesterol 7alpha-hydroxylase / 25-hydroxyvitamin D_3 1alpha-hydroxylase / enzyme activity / substrate specificity / bile acids / vitamin D_3
Research Abstract

1. Assay of human cholesterol 7alpha-hydroxylase
Microsomal cholesterol 7alpha-hydroxylase activity was not assayed as an increased measurement when we measured it after solubilizing the microsomes and reconstituting the P450 reaction system ideally ; whereas mitochondrial cholesterol 27-hydroxylase activity was assayed as a markedly increased measurement when we measured it after solubilizing the mitochondria and reconstituting the P450 reaction system ideally.
The results will be published.
2. Cholesterol and vitamin D_3 metabolisms
Cholesterol 27-hydroxylase and vitamin D_3 25-hydroxylase are performed by one common enzyme.
To survey these phenomena, I focused to study enzymes concerning vitamin D_3. First, we purified 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria, and published as two papers.
Substrate specificity of the enzyme will be studied in future.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Nakamura,Y.,・・・Setoguchi,T.,et al: "Purification and characterization of 25-hydroxyvitamin D_31α-hydroxylase from rat kidney mitochondria." FEBS Letters. 419(1). 45-48 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eto,T.-A.,・・・Setoguchi,T.,et al: "Assay of 25-hydroxyvitamin D_31α-hydroxylase in rat kidney mitochondria." Analytical Biochemistry. 258. 53-58 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura,A.,・・・Setoguchi,T.,et al: "Diagnosis of the first Japanese patient with 3-oxo-Δ^4-steroid 5β-reductase deficiency by use of immunoblot analysis." European Journal of Pediatrics. 157. 386-390 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, Y., Eto, T.-A., Taniguchi, T., Miyamoto, K., Nagatomo, J., Shiotsuki, H., Sueta, H., Higashi, S., Okuda, K.-I.and Setoguchi, T.: "Purification and characterization of 25-hydroxyvitamin D_3 1alpha-hydroxylase from rat kidney mitochondria." FEBS Letters. 419(1). 45-48 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eto, T.-A., Nakamura, Y., Taniguchi, T., Miyamoto, K., Nagatomo, J., Maeda, Y., Higashi, S., Okuda, K.-I.and Setoguchi, T.: "Assay of 25-hydroxyvitamin D_3 1alpha-hydroxylase in rat kidney mitochondria." Analytical Biochemistry. 258. 53-58 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, A., Kondo, K.-H., Okuda, K.-I., Higashi, S., Suzuki, M., Kurosawa, T., Tohma, M., Inoue, T., Nishiyori, A., Yoshino, M., Kato, H,and Setoguchi, T.: "Diagnosis of the first Japanese patient with 3-oxo-DELTA^4-steroid 5beta-reductase deficiency by use of immunoblot analysis." European Journal of Pediatrics. 157. 386-390 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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