1998 Fiscal Year Final Research Report Summary
The research for the developement and progression in hepato-biliary-pancreatic carcinoma
| Project/Area Number |
08457330
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | NARA MEDICAL UNIVERSITY |
|---|
Principal Investigator |
NAKANO Hiroshige Nara Medical University, Faculty of Medicine, Professor, 医学部, 教授 (20075071)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HISANAGA Michiyoshi Nara Medical University, Faculty of Medicine, Associate Professor, 医学部, 講師 (30275341)
KANEHIRO Hiromichi Nara Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30204580)
NAKAJIMA Yoshiyuki Nara Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00142381)
|
|---|
| Project Period (FY) |
1996 – 1998
|
| Keywords | Fas / FasL / apoptosis / angiogenesis / VEGF / PD-ECGF / MRP-1 / CD9 / KAI-1 / CD82 |
|---|
| Research Abstract |
We analyzed the expression of p21, p53 and PCNA in chronic liver disease and HCCs. Immunohistochemical assay showed close correlation between the level of p21 expression and PCNA LI in noncancerous liver tissues. In HCCs, p21 overexpressing tumors showed high PCNA LI and smaller size. Clinically, p21 overexpressing, p53 negative and low PCNA LI cases showed high disease-free survival rate. The alteration of Fas/FasL system including soluble forms is regarded as one of mechanisms preventing the immune system from rejecting tumor cells. We studied expression of Fas and FasL and serum levels of sFas and sFasL in HCC.The reduction of Fas expression was associated with intrahepatic metastasis, low apoptotic
cells, the poor porgnosis and p53 positivity. sFas and sFasL levels in HCC patients were significantly higher and lower than those in controls, respectively.
To clarify the relationship between transmembrane 4 superfamily (TM4SF) and the clinicopathological findings of pancreatic cancer, MRP-l/CD9 and KAI1/CD82 expression was evaluated in pancreatic adenocarcinomas. MRP- 1/CD9 gene expression was associated with lymph node status, pathological status and histo-pathological grading. KAI1/CD82 gene expression was associated with tumor status. Clinically, MRP-l/CD9-positive cases and KAI1/CD82-positive cases showed high survival rate.
To evaluate whether angiogenic factors are of clinical relevance to human pancreatic cancers, we studied the intratumoral microvessel density (iMD), and PD-ECGF, VEGF expression in pancreatic cancers. VEGF was moderately associated with an increase in the IMD, but no significant relationship was found between PD-ECGF and the iMD.However, tumours with positive expression for both PD-ECGF and VEGF had a higher IMD.Clinically, patients with hypervascular, PD-ECGF positive and VEGF positive tumors showed poor prognosis.
|
