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1998 Fiscal Year Final Research Report Summary

Development and clinical evaluation of a new lipid microsphere-encapsulated antifungal drug.

Research Project

Project/Area Number 08457331
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionWakayama Medical Scool

Principal Investigator

TANIMURA Hiroshi  Wakayama Medical School Professor, 医学部, 教授 (10026990)

Co-Investigator(Kenkyū-buntansha) TSUNODA Takuya  Wakayama Medical School Assistant, 医学部, 助手 (30275359)
UCHIYAMA Kazuhisa  Wakayama Medical School Lecturer, 医学部, 講師 (80232867)
Project Period (FY) 1996 – 1998
KeywordsAmphotericin / lipid-Microsphere / Candida albicans / Abdominal fungal abscess / Candidemia / PCR / competitive PCR / fungal translocation
Research Abstract

Incorporating AMPH into lipid emulsion(AMPH-lipid microsphere), wich if prepared by emulsification, was investigated for its structure and evaluated for its toxicity in vivo and in votro, and its efficacy in invasive general candidiasis in BALBc mice. The diameter of AMPH-lipid microsphere was small and stable during 90 days. It seemed that AMPH could be located in the phospholipid layer in the lipid complex formation. In vitro, AMPH-lipid rnicrosphere was less toxic aganisl hemolysis than the conventional AMPH(Fungizon). In vivo, AMPH-lipid microsphere was markedly less toxic in the survival rate of mice. It have shown an increased therapeutic index resulting in higher dose-dependent efficacy mice with systemic a new of model of local intraperitoneal candidasis. These findings suggested that incorporating AMIPH into lipid emulsion might be advantageous for treatment of candidiasis in surgery.
In the present study, a Candida specific PCR was performed to analyze by using the specific primer for V4 region of 18s gene of Candida species. To quantify the Candida, new heterogenous DNA competitor(comp V4 : 227bp) was newly made, and competitive PCR was periformed. Then it clarified that quantitiative measurement from PCR product was posible to analyze under the fg level of Candida-DNA.
It indicated that via portal vein was one pathway of fungal translocation, because Candida was detected by PCR from the portal venous blood during operatoin. Thus the Candida specific PCR may be an excellent tool for early diagnosis of deep mycosis clinically and for further understanding of fungal translocation.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 谷村 弘: "新抗菌薬臨床評価の現状と問題点-新しいガイドラインの位置付け. 外科領域感染症" 日本化学療法学会雑誌. 45(9). 792-796 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷村 弘: "成分分析からみた肝内結石の成因" 胆道. 11(3). 237-248 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内山和久: "日本人胆石の特徴と無症状胆石" 胆と膵. 19(4). 275-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内山和久: "内視鏡的乳頭括約筋切開術" 消化器外科. 21(5). 798-801 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 角田卓也: "腫瘍免疫-最近の進歩ペプタイドと腫瘍免疫「臨床編」" 癌治療と宿主. 10(1). 99-104 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 角田卓也: "ペプタイドを用いる癌特異的免疫療法の飛躍的成績向上のための新戦略" Biotherapy. 12. 578 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷村 弘: "外科と栄養. 栄養・健康科学シリーズ, 臨床栄養学" 南江堂, 東京, 14 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷村 弘: "起炎菌からみた術後感染症対策. MRSA" 南山堂, 東京, 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanimura H: "The present circumstances and problems in clinical evaluation of new antibacterial dlugs" Jpn.J.Chemother. 45. 792-796 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanimura H: "The cause of the hepatolithiasis from a component analysis." Biliary Tract. 11. 237-248 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama K: "The features of Japanese cholecystolithiasis and asymptomatic gall stone" Tan to Sui.19. 275-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama K: "Endoscopic sphincterotomy" Gastroenterogy. 21. 798-801 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunoda T: "Tumor immunity-Recent advances of clinical trials for cancer vaccine" Cancer Treatment and Host. 10. 99-104 (1998)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunoda T: "New strategy for tumor specific immunotherapy using tumor antigen peptides" Biotherapy. 12. 578 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita H: "Investigation of new lipid microsphere-encapsulated antifungal drug" J.Wakayama Med.Soc.49. 153-165 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kontani T: "Establishment of PCR-diagnosis for Candida albicans and clinical investigation for fungal translocation" J.Wakayama Med.Soc. 49. 209-222 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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