1998 Fiscal Year Final Research Report Summary
Development and clinical evaluation of a new lipid microsphere-encapsulated antifungal drug.
| Project/Area Number |
08457331
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Wakayama Medical Scool |
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Principal Investigator |
TANIMURA Hiroshi Wakayama Medical School Professor, 医学部, 教授 (10026990)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNODA Takuya Wakayama Medical School Assistant, 医学部, 助手 (30275359)
UCHIYAMA Kazuhisa Wakayama Medical School Lecturer, 医学部, 講師 (80232867)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Amphotericin / lipid-Microsphere / Candida albicans / Abdominal fungal abscess / Candidemia / PCR / competitive PCR / fungal translocation |
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| Research Abstract |
Incorporating AMPH into lipid emulsion(AMPH-lipid microsphere), wich if prepared by emulsification, was investigated for its structure and evaluated for its toxicity in vivo and in votro, and its efficacy in invasive general candidiasis in BALBc mice. The diameter of AMPH-lipid microsphere was small and stable during 90 days. It seemed that AMPH could be located in the phospholipid layer in the lipid complex formation. In vitro, AMPH-lipid rnicrosphere was less toxic aganisl hemolysis than the conventional AMPH(Fungizon). In vivo, AMPH-lipid microsphere was markedly less toxic in the survival rate of mice. It have shown an increased therapeutic index resulting in higher dose-dependent efficacy mice with systemic a new of model of local intraperitoneal candidasis. These findings suggested that incorporating AMIPH into lipid emulsion might be advantageous for treatment of candidiasis in surgery.
In the present study, a Candida specific PCR was performed to analyze by using the specific primer for V4 region of 18s gene of Candida species. To quantify the Candida, new heterogenous DNA competitor(comp V4 : 227bp) was newly made, and competitive PCR was periformed. Then it clarified that quantitiative measurement from PCR product was posible to analyze under the fg level of Candida-DNA.
It indicated that via portal vein was one pathway of fungal translocation, because Candida was detected by PCR from the portal venous blood during operatoin. Thus the Candida specific PCR may be an excellent tool for early diagnosis of deep mycosis clinically and for further understanding of fungal translocation.
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