1998 Fiscal Year Final Research Report Summary
Regeneration of injured central nervous system by cell therapy using neural cell adhesion molecule L1
| Project/Area Number |
08457362
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
ARITA Norio Osaka University Medical School, Lecturer, 医学部, 講師 (80159508)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Kensaku National Institute of Bioscience and Human Technolgy, Director, フロンティア研究システム・ニューロン機能研究グループ, ディレクター (60008563)
IZUMOTO Shuichi Osaka University Hospital, Medical staff, 医学部・附属病院, 医員
OHNISHI Takanori Osaka University Medical School, Assistant Professor, 医学部, 助手 (70233210)
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| Project Period (FY) |
1996 – 1998
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| Keywords | L1 / cell adhesion molecule / nerve regeneration / optic nerve / nerve injury / visual evoked potential |
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| Research Abstract |
Optic nerve has been believed not to regenerate in postnatal mammals except if peripheral nerve transplants are offered as a substrate or if treated with transglutaminase. Neural cell adhesion molecule L1 is a member of the immunoglobulin superfamily. L1 plays important roles in neuron-neuron adhesion, neurite fasciculation, neurite outgrowth and neural cell migration. The homophilic binding of L1 in neuronal growth cones to L1 expressed on the surface of the other cells with or without the assistance of NCAM can promote axonal growth. We. Present evidence that -the transected optic nerve in adult rats was promoted to. Regenerate by transplanted L1-expressing cells that were embedded in the Matigel matrix. To obtain the maximum effects, a very careful operative procedure by which both stumps remained attached and the blood supply was preserved, was essential. Visual evoked potential was partially recovered and traced fibers were seen to pass through the lesion 7 weeks after optic nerve transection. Immunohistochemical analysis demonstrated that neurofilament-positive fibers were ; present around the site of experimental lesion. These results present electrophysiological and morphological evidences that the optic nerv& in adult rats has the potential to regenerate if the surrounding matrix contains L1 presenting cells without using peripheral nerve grafts.
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