1998 Fiscal Year Final Research Report Summary
Mechanism of iontransports in the cells of the auditory system.
| Project/Area Number |
08457453
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Miyazaki Medical College (1997-1998)
Kyushu University (1996) |
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Principal Investigator |
KOMUNE Shizuo Miyazaki Medical College, Dept.of Otolaryngol., Chairman, 医学部, 教授 (10117434)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Endocochlear potential / Na-K-Cl contransport / endolymph / Cochlea |
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| Research Abstract |
Ouabain(1mM) applied in the perilymphatic space of the guinea-cochlea decreased the endolymphatic potential(EP) to the maximum negative level of approximately 30 mV and also did the potassium activity of endolymph([K]e). After EP reached the maximum negative potential , the perilymphatic space was perfused with Cl-free, Na-free, or 2 mM of furosemide containing 1mM of ouabait in each solution. Cl-free and Na-free inhibited the decrease of [K]e due to 1 mM of ouabain. 2mM of furosemide also inhibited the decrease of [K]e. EP did not changed during these experiments. These findings is an evidence that there exists a Na-K-Cl cotransporter in the in vivo cocblea, which is directed toward from perilymph to endolymph, ouabain-insennsitive and neutral electrically.
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[Publications] Kimitsuki, T., Nakagawa, T., Hisashi, K. Tsuji, K., Komune, S., Komiyama, S.: "Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell." Acta Otolaryngol(Stockh). 118. 70-73 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kimitsuki, T., Nakagawa, T., Hisashi, K., Tsuji, K., Komune, S., komiyama, S.: "Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell." Acta Otolaryngol (Stockh). 118. 70-73 (1998)
Description
「研究成果報告書概要(欧文)」より
