1997 Fiscal Year Final Research Report Summary
Isolation and identification of cDNAs related with mouse tooth germs and its localization on human odontogenic tumor.
| Project/Area Number |
08457482
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
OKABE Haruo Nagasaki University, School of Dentistry, Professor, 歯学部, 教授 (10005019)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Yasuaki Nagasaki University, School of Dentistry, Research assistant, 歯学部, 助手 (80253673)
FUJITA Shuichi Nagasaki University, School of Dentistry, Research assistant, 歯学部, 助手 (00181355)
TAKAHASHI Hiroshi Nagasaki University, School of Dentistry, Assistant professor, 歯学部, 助教授 (20124597)
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| Project Period (FY) |
1996 – 1997
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| Keywords | TOOTH GERM / IN SITU HYBRIDIZATION / RT-PCR / T-T DIMER |
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| Research Abstract |
We isolated cDNAs from excised tooth germs and giniva of mouse embryo (E13-E17) and compared expression of cDNAs of each tissue by using RT-PCR and differential display. The eluted PCR products of tooth germ-spcecific cDNAs from acrylamide gels were thymine-thymine (T-T) dimerized by ultra violet irradiation. The specificity of the cDNAs were confirmed by in situ hybridization applied to tooth germs of mouse embryo (E14-17) by using the each T-T dimerized cDNA probe. By this method, we isolated mouse amelogenin cDNA and the coding region had 83% identity with human amelogenin.
Moreover, we performed in situ hybridization for the same amelogenin cDNA probe on human odontogenic tumor : Ameloblastoma (plexiform type, follicular type and mixed type), Calcifying odon togenic tumor, and Cementoma. The amelogenin was detected on ameloblastoma both plexiform and follicular type but not calcifying odontogenic tumor and cementoma, because of previous decalcification by hydrochloric acid.
These results reveals that the in situ hybridization using T-T dimerized cDNA probe were available for screening and isolation of novel tissue specific mRNA.
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