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1998 Fiscal Year Final Research Report Summary

Molecular mechanisms of ion channels and second messengers in gustatory transduction of taste cells.

Research Project

Project/Area Number 08457491
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

SATO Toshihide  Nagasaki University, School of Dentistry, Professor, 歯学部, 教授 (60013968)

Co-Investigator(Kenkyū-buntansha) FUJIYAMA Rie  Nagasaki University, School of Dentistry, Research Associate, 歯学部, 助手 (10274664)
MIYAMOTO Takenori  Nagasaki University, School of Dentistry, Research Associate, 歯学部, 助手 (10167679)
OKADA Yukio  Nagasaki University, School of Dentistry, Associate Professor, 歯学部, 助教授 (60136687)
Project Period (FY) 1996 – 1998
Keywordstaste cell / patch clamp technique / IP_3 / cAMP / mouse / gerbil / transduction / K^+ channel
Research Abstract

1. When inward currents in the fungiform papilla taste cells induced by NaCi stimuli were analysed, it was found that the taste cells in the mouse were divided into two types according to the presence and absence or almost absence of amiloride-blockable epithelial Na^+ channels (ENaC) in the apical receptive membrane. When large inward currents elicited by NaCi stimuli were blocked by amiloride, the subunits of ENaC may be composed of alpha, beta and gamma. However,
when the inward currents were not blocked completely or almost completely by amiloride, it is likely that the apical membrane of a taste cell lacks the whole subunits or beta and gamma of ENaC.
2. The inward current in the mouse induced by an acid stimulus was not blocked at all by amiloride. Therefore, the acid response of mouse taste cells is not evoked via the amiloride-blockable ENaC.Our recent finding suggests that there are two pathways for generation of acid responses. One is a cascade of binding of proton to ionotoropic receptor in the apical membrane and inflow of cations through the cation channels. The other pathway is a cascade of activation of G-protein and effector molecule, production of a second messenger and closure of C1^- channels in the basolateral membrane via a protein kinase.
3. Two pathways are concerned with generation of sweet responses in gerbil taste cells for two types of sweeteners. One pathway for sugar stimuli is a cascade of activation of G-protein and adenylate cyclase, production of cAMP and closure of K^+ channels via a protein kinase. The other pathway for non-sugar sweeteners is a cascade of activation of G-protein and PLC, production of 1P3 and release of Ca^2^+ from the intracellular Ca^2^+ store.

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Miyamoto,T.,et al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J.Neurosci.Methods. 64. 245-252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto,T.,et al.: "Properties of Na^+-dependent K^+ conductance in the apical membrane of frog taste cells." Brain Res.715. 79-85 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,Y.,et al.: "Vasopressin modulates membrane properties of taste cells isolated from bullfrogs." Chem.Senses. 21. 739-745 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida,Y.,et al.: "Intracellular calcium increase in gerbil taste cell by amino acid sweeteners." Chem.Senses. 22. 83-91 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato,T.,et al.: "Broad tuning of rat taste cells for four basic taste stimuli." Chem.Senses. 22. 287-293 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida,Y.,et al.: "Changes in outward K^+ currents in response to two types of sweeteners in sweet taste transduction of gerbil taste cells." Chem.Senses. 22. 163-169 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato,T.,et al.: "Distribution of non-tasters for phenylthiocarbamide and high sensitivity to quinine hydrochloride of the non-tasters in Japanese" Chem.Senses. 22. 547-551 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okada,Y.,et al.: "Inositol 1,4,5-trisphosphate activates non-selective cation conductance via intracellular Ca^<2+> increase in isolated frog taste cells." Eur.J.Neurosci.10. 1376-1382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiyama,R.,et al.: "Intracellular free calcium concentration in human taste bud cells increases in response to taste stimuli." FEBS Lett.434. 47-50 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto,T.,et al.: "Sour transduction involves activation of NPPB-sensitive conductance in mouse taste cells." J.Neurophysiol.80. 1852-1859 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto,T.,et al.: "Salty and sour transduction:Multiple mechanisms and strain differences." Olfaction and Taste XII. 128-133 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 星 猛 ら: "医科生理学展望 原書第17版" 丸善, 828 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高田 明和 ら: "アトラスで学ぶ生理学" 丸善, 281 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 昌康 ら: "最新 味覚の科学" 朝倉書店, 251 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 星 猛 ら: "医科生理学展望 原書第18版" 丸善, 851 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto, T., et al.: "Whole-cell recording from non-dissociated taste cells in mouse taste bud." J.Neurosci.Methods. 64. 245-252 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, T., et al.: "Properties of Na^+ -dependentK ^+ conductance in the apical membrane of frog taste cells." Brain Res.715. 79-85 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, Y, , et al.: "Vasopressin modulates membrane prooerties of taste cells isolated from bullfrogs." Chem.Senses. 21. 739-745 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchida, Y., et al.: "Intracellular calcium increase in gerbil taste cell by amino acid sweeteners." Chem.Senses. 22. 83-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, T., et al.: "Broad tuning of rat taste cells for four basic taste stimuli." Chem.Senses. 22. 287-293 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchida, Y., et al.: "Changes in outward K^+ currents in response to two types of sweeteners in sweet taste transduction of gerbil taste cells." Chem.Senses. 22. 163-169 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, T., et al.: "Distribution of non-tasters for pheylthio-carbamide and high sensitivity to quinine hydrochloride of the non-tasters in Japanese." Chem.Senses. 22. 547-551 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, Y., et al.: "Inositol 1,4,5-trisphosphate activates non-selective cation conductance via intracellular Ca^<2+> increase in isolated frog taste cells." Eur.J.Neurosci. 10. 1376-1382 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiyama, R., et al.: "Intracellular free cslcium concentration in human taste bud cells increases in response to taste stimuli." FEBS Lett.434. 47-50 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, T., et al.: "Sour transduction involvoes activation of NPPB-sensitive conductance in mouse taste cells." J.Neurophysiol.80. 1852-1859 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, T., et al.: "Salty and sour transduction : Multiple mechanisms and strain differences." Olfaction and Taste XII. 128-133 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hoshi, T., et al.: Review of Medical Physiology (17ed.). Maruzen, 828 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takada, A., et al.: Physiology Studying with Atlas.Maruzen, 281 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, M., et al.: Recent Gustatory Science.Asakura, 251 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hoshi, T., et al.: Review of Medical Physiology (18 ed.). Maruzen, 851 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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