1998 Fiscal Year Final Research Report Summary
Participation of c-fosgene with pathogenesis of periodontitis
| Project/Area Number |
08457510
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Showa University |
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Principal Investigator |
HASEGAWA Kohoji Showa University, Dental school Professor, 歯学部, 教授 (70014024)
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| Co-Investigator(Kenkyū-buntansha) |
SHIONOME Manabu Showa University, Dental school Assistant, 歯学部, 助手 (50245809)
MIYAGAWA Yasushi Showa University, Dental school Lecture, 歯学部, 講師 (90219775)
KOBAYASHI Makoto Showa University, Dental school Lecture, 歯学部, 講師 (80186767)
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| Project Period (FY) |
1996 – 1998
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| Keywords | periodontitis / c-fosgene / human gingibal fiibroblasto / interleukin-1 / cell growth / intercellular 1L-1αproduction / prostaglandin EィイD22ィエD2 production |
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| Research Abstract |
Previous reports demonstrated that c-fos gene participates with cell growth and differentiation, and this may play an important role in intracellular signaling cascade of inflammatory cytokines and lipopolisaccharide. Interleukin-1 (IL-1) stimulates local inflammation through enhancement of inflammatory factors' production and induces destraction of periodontal tissues. The purpose of this study was to clarify a participation of c-fos gene with pahthogenesis of periodontitis. In this study, we investigated that the participation of c-fos gene with IL-1-induced cell growth and production of intracellular IL-1α and prostaglandin EィイD22ィエD2(PGEィイD22ィエD2) in human gingival fibroblasts (HGF). Results obtained as follows : (1) IL-1β stimulated c-fos mRNA expression more rapidly than expression of IL-1α, cyclooxygenase-2(COX-2) and cytosolic phospholipase AィイD22ィエD2 (cPLAィイD22ィエD2) mRNA. (2) IL-1β-induced cell growth, expression of IL-1α, COX-2 and cPLAィイD22ィエD2 mRNA and synthesis of intracellular IL-1α and PGEィイD22ィエD2 were partially inhibited by co-treatment with c-fos antisense ODN. (3) Co-treatment with curcumin, a inhibitor of activating protein-1 (AP-1), suppressed IL-1β-induced IL-1α, COX-2 and cPLAィイD22ィエD2 mRNA expression and intracellular IL-1α and PGEィイD22ィエD2 synthesis. These results demonstrated that c-fos gene may play a principal role in cell growth and production of intracellular IL-1α and PGEィイD22ィエD2 in human gingival fibroblasts stimulated with IL-1β, and these suggested a participation of c-fos gene with pahthogenesis of periodontitis.
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