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1998 Fiscal Year Final Research Report Summary

ESTABLISHMENT OF MOLECULAR DIAGNOSING STRATEGY FOR MALIGNANT TRANSFORMATION OF ORAL LEUKOPLAKIA

Research Project

Project/Area Number 08457539
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

NOTANI Ken-ichi  SCHOOL OF DENTISTRY,HOKKAIDO UNIVERSITY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (70113602)

Co-Investigator(Kenkyū-buntansha) SHINDOH Masanobu  SCHOOL OF DENTISTRY,HOKKAIDO UNIVERSITY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (20162802)
CHIBA Itsuo  SCHOOL OF DENTISTRY,HOKKAIDO UNIVERSITY,INSTRUCTOR, 歯学部, 助手 (50250460)
Project Period (FY) 1996 – 1998
KeywordsORAL SCC / PREMALIGNANT LESION / P53 GENE / MASA / YEAST FUNCTIONAL ASSAY / MICROSATELLITE / SRI LANKA / BETEL CHEWING
Research Abstract

Carcinogenesis is a multistep process, and thus an accumulation of several independent genetic abnormalities is necessary for the progression of cancer. Recent research on genetic alterations found in human cancers has shown that activation of oncogenes and inactivation of tumor suppressor genes are major steps in the development of cancers. Mutations of the p53 gene have also been observed in a variety of non-invasive, dysplastic epithelial lesions from various tissues, including oral cavity, esophagus, and lung. Since premalignant legions could be genetically heterogenous, it is necessary to use sensitive methods for detecting mutations in a small population. Because of this background, we analyzed genetic changes occurring in human oral SCC and premalignant lesions in Japan and Sri Lanka, which were caused by betel chewing, to clarify specific genetic events for promoting premalignant lesion to cancer (Oncogene, 15 : 2667, 1997., The 90th Annual Meeting of American Association for Cancer Research). In addition, we used DMBA-induced hamster cheek pouch carcinogenesis model to analyze mutations in the p53 and ras genes chronologically (Jpn. J.Oral Maxillofac. Surgery, 42 : 347, 1996., ibid, 44 : 925, 1998.). The following methods were applied ; 1) MASA (mutant allele specific amplification), 2) yeast functional assay, and 3) analysis of microsatellite alteration. Mutations in the p53 gene, the ras gene and microsatellite alterations occurred not only in cancers but also in premalignqant lesions in both animal model and human cases. Our data suggest that genetic changes could occur during premalignant stages and it is important to have preventive strategies during these stages.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Itsuo Chiba, et al.: "Mutations in the p53 gene and human papillomavirus infection as significant prognostic factors in squamous cellcarcinomas of the oral cavity." Oncogene. 12. 1663-1668 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haruhiko Kashiwazaki, et al.: "High frequency of p53 mutations in human oral epithelial dysplasia and primary squamous cell carcinoma detected by yeast functional assay." Oncogene. 15. 2667-2674 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itsuo Chiba, et al.: "Characteristics of mutations in teh p53 gene of oral squamous cell carcinomas associated with betel quid chewing in Sri Lanka." International Journal of Cancer. 77. 839-842 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山崎 裕, 他: "MASA法を用いた実験的口腔癌の発癌過程におけるH-ras遺伝子変異の検出." 日本口腔外科学会雑誌. 44. 925-930 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山崎 裕, 他: "DMBA誘発ハムスター頬嚢粘膜癌のfield cancerizationモデルにおけるp53遺伝子変異の多様性." 日本口腔科学会雑誌. 47. 316-322 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itsuo Chiba: "Mutations in the p53 gene and human papillomavirus infection as significant prognostic factors in squamous cellcarcinomas of the oral cavity." Oncogene. 12. 1663-1668 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Haruhiko Kashiwazaki: "High frequency of p53 mutations in human oral epithelial dysplasia and primary squamous cell carcinoma detected by yeast functional assay." Oncogene. 15. 2667-2674 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itsuo Chiba: "Characteristics of mutations in the p53 gene of oral squamous cell carcinomas associated with betel quid chewing in Sri Lanka." International Journal of Cancer. 77. 839-842 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Yamazaki: "Application of mutant allele specific amplification (MASA) for detecting H-ras mutations during DMBA-induced hamster cheek pouch carcinogenesis." Japanese Journal of Oral & Maxillofacial Surgery. 44. 925-930 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yutaka Yamazaki: "Variation of p53 mutations in field cancerization model of DMBA-induced SCC of hamster cheek pouch" Journal of the Japanese Stonatological Society. 47. 316-322 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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