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1997 Fiscal Year Final Research Report Summary

Cell cycle and apoptosis in the process of carcinogenesis in oral precancerous lesions

Research Project

Project/Area Number 08457554
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionShowa University

Principal Investigator

NAGUMO Masao  Showa university, School of Dentistry, Professor, 歯学部, 教授 (70013993)

Co-Investigator(Kenkyū-buntansha) KAMIJO Ryutaro  Showa University, School of Dentistry, Assistant Professor, 歯学部, 講師 (70233939)
IWASA Masayasu  Showa University, School of Dentistry, Assistant Professor, 歯学部, 講師 (50193743)
Project Period (FY) 1996 – 1997
Keywordsoral leukoplakia / G1 cycline / cycline dependent kinase / cell cycle / p21 / waf1 / p53 knockout mouse / TUNEL method / apoptosis
Research Abstract

Expression of G1 cyclins, cyclin dependent kinases (CDK) and p21/waf1 was examined in oral leukoplakia (OLP) snd oral cancer tissue. In addition, growth activity and cell cycle of oral mucosal keratinocytes in p53 gene knockout mouse were also studied.
The results obtained were as follows :
1.Cyclin D1, cyclin E,CDK2 and CDK4 were weekly expressed in normal oral mucosal epithelium, whereas their expression in OLP became strong with the grade of epithelial dysplasia. Each positivity was significantly higher in OLP with moderate and severe epithelial dysplasia and oral cancer tissues than in normal oral mucosal epithelium.
2.p21/waf1 was not expressed in normal oral mucosal epithelium and oral cancer tissue, but it was found in OLP with moderate and severe epithelial dysplasia. However, p21/waf1 mRNA was expressed in all tissues.
3.TUNEL-positive cells were most frequently observed in normal oral mucosal epithelium. Decreasing tendency in TUNEL-positive cell rate was found with the grade of epithelial dysplasia and carcinogenesis.
4.Growth activity of oral mucosal keratinocytes in p53 knockout mouse was higher than that in normal (wild) mouse. The flowcytometric analysis revealed that the duration of G1 phase was shortened in p53 knockout mouse.
These results suggest that the misrule of cell cycle and suppression of apoptosis may be concerned in carcinogenesis of OLP.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 中村 雅子 他: "白板症の免疫組織化学的検討・上皮異形成とTGFL発現との関係" 日本口腔粘膜学会雑誌. 2(1). 49-53 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 油井 久美恵 他: "口腔白板症における喫煙と細胞増殖マーカーとの関連について" 日本口腔粘膜学会雑誌. 2(2). 90-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumitani K. et al.: "Cytotoxic effect of sodium nitroprusside on Cancercells : Inoolcement of apoptosis and supression of C-Myc and C-MYB protoncogene oxpression" Anticancer Research. 17(2). 865-872 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura M. et al.: "Immunohrstochemicol study on oral leukoplakia-Correlotion of epithelial dysplasia with hey antigen and p53 profeln." Dentistry in Japan. 3(4)(in-press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 油井 久美恵: "口腔白板症におけるG1サイクリンおよびサイクリン依存性キナーゼの発現" 日本口腔粘膜学会雑誌. 4(1)(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito D. et al.: "Altered groroth response of orol mucasol keralinocytos in p53-deficient mice." J Oral Pothol Oral Med. 27(in-press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masako Nakamura, Kumie Yui, Daisuke Ito, Atusi Saito, Saburo Kakuta and Masao Nagumo: "Immunohistochemical study on oral leukoplakia-Correlation of epithelial dysplasia with TGF-alpha-" J Jpn Oral Muco Membr. 2(1). 49-53 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumi Yui, Masako Nakamura, Toshie Sakata, Toshiaki Takemi, Atusi Sato, Saburo Kakuta, Yoichi Kurachi and Masao Nagumo: "Relationship between smoking and cell proliferating marker in oral leukoplakia." J Jpn Oral Muco Memr. 2(2). 90-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaname Sumitani, Ryutaro Kamijo and Masao Nagumo: "Cytotoxic effect of sodium nitroprusside on cancer cells : Involvement of apoptosis and suppression of C-MYC and C-MYB proto-oncogene expression." Anticancer Res. 17(2). 865-872 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masako Nakamura, Keiko Kanemoto, Kumie Yui, Saburo Kakuta, Yoichi Kurachi and Masao Nagumo: "Immunohistochemical study on oral leukoplakia-Correlation of epithelial dysplasia with Le^y antigen and p53 protein-" Dentistry in Japan. 34(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumi Yui: "Expression of G1 cyclins and cyclin dependent Kinases in oral leukoplakia." J Jpn Oral Muco Membr. 4(1)(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Daisuke Ito, Ryutaro Kamijo, Kunio Takizawa, Kaname Sumitani and Masao Nagumo: "Altered growth response of oral mucosal keratinocytes in p53-deficient mice" J Oral Pathol Oral Med. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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