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1997 Fiscal Year Final Research Report Summary

Changes of DNA supercoiling in Escherichia coli induced by stress

Research Project

Project/Area Number 08457611
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SEKIMIZU Kazuhisa  Faculty of Pharmaceutical Sciences, Kyshu University Professor, 薬学部, 教授 (90126095)

Project Period (FY) 1996 – 1997
Keywordsheat shock proteins / DNAsupercoling DNA gyrase / sigma 32 / DNA-dependent protein synthesis / DNA依存蛋白合成系
Research Abstract

We previously reported that plasmid DNA in Escherichia coli relaxd when cells were exposed to stress. In this study, we examined stability and synthesis of sigma 32 in the presence of inhibitors of DNA gyrase.The result showed that the inhibitors stabilized sigma 32, and stimulated the synsthsis of the protein. We also showed that DNA relaxation stimulated synthesi of simga 32 in an in vitor system of DNA-dependent protein synthesis. Taken together, we conclude that DNA relaxation induces stability and synthesis of sigman 32 in cells. We propose that changes of DNA supercoiling in cells is a key signal for induction of heat shock proteins.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Mizushima, T., Kataoka, K. Miki, T., and Sekimizu, K.: "Increase in negative supercoilingof plasmid DNA in Escherichia coli exposed to cold shock." Molecular Microbiology. 23. 381-386 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizushima, T., Kataoka, K., Miki, T., and Sekimizu, K.: "Increase in negative supercoiling of plasmid DNA in Escherichia coli exposed to cold shock." Mol.Microbiol.23. 381-386 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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