1997 Fiscal Year Final Research Report Summary
Membrane Protein Recognition by Ladder-shaped Polyether Compounds
| Project/Area Number |
08458171
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioorganic chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
MURATA Michio The University of Tokyo, Graduate School of Science, Associate Professor, 大学院・理学系研究科, 助教授 (40183652)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SASAKI Makoto The University of Tokyo, Graduate School of Science, Research Associate, 大学院・理学系研究科, 助手 (80235267)
TACHIBANA Kazuo The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (70142081)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Keywords | polyether compounds / membrane proteins / maitotoxin / ciguatoxin |
|---|
| Research Abstract |
Marine microalgae are known to be arich source of structurally and biologically intriguing polyketides. Among those, maitotoxin (MTX) attracts attention of chemists and biochemists since the toxin possesses the largest molecular weight of 3422 Da among secondary metabolites and very potent lethality (50 ng/kg, mice, ip.). Its structure has been determined by combined use of NMR,MS/MS and synthetic chemistry. A new method based on carbon-proton spin coupling (2,3JC,H) was introduced for the configurational determination of the acyclic sidechains. Based on these data, the stereostructure of the whole molecule has been elucidated, which sheds light on its molecular mode of action. Recent findings suggest that MTX recognaizes its taget protein with the hydrophobic polycyclic ethers (the upper half of the structure), which probably interacts with the transmembrane portion of the protein.
|
