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1997 Fiscal Year Final Research Report Summary

Molecular Anatomy of RNA Polymerase : Identification of Transcription Factor Contact Sites

Research Project

Project/Area Number 08458224
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionNational Institute of Genetics

Principal Investigator

ISHIHAMA Akira  National Institute of Genetics, Department of Molecular Genetics, Professor and Head, 分子遺伝研究系, 教授 (80019869)

Co-Investigator(Kenkyū-buntansha) FUJITA Nobuyuki  National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (90173434)
Project Period (FY) 1996 – 1997
KeywordsTranscription regulation / Transcription apparatus / Molecular interaction / Protein-DNA interaction / RNA polymerase / Artificial protease / Artificial nuclease
Research Abstract

The RNA polymerase holoezyme of Escherichia coli is composed of core enzyme with the subunit structure of alpha_2betabeta' and one of seven different molecular species of sigma subunits, each recognizing a different promoter sequence. The promoter selectivity of RNA polymerase is further modulated after interaction with one or two of more than 100 different species of trans-acting protein and nucleotide factors, generally designated as transcription factors, with the regulatory activities of transcriprion. The RNA polymerase activity is also modulated by cis-acting regulatory DNA signals generally located upstream of promoters. The contact sites on RNA polymerase subunits have been determined for more than 30 transcription regulatory protein or nucleotide factors. Based on the contact sites, we proposed to classify the transcription factors into four groups : clss-I(alpha contact), II(sigma contact), III(beta contact) and VI(beta' contact) factors. The contact sites estimated from mutant studies have been confirmed using a new tool of chemical reagent, Fe-BABE,with contact-dependent cleavage activity of DNA and proteins.
Since the total number of RNA polymerase core enzyme is fixed at a low and constant level characteristic of the rate of cell growth, the concentrations of each from holoenzyme and ezch RNA polymerase-transcription factor complex are subject to fluctuation depending on growth-dependent variations in the concentrations of each sigma subunit and each transcription factor. Variation in the molecular arhitecture of transcription apparatus was analyzed during growth transition of E.coli from exponentially growing to stationary phase. Accordingly, the species and order of gene transcription among about 4,000 genes on the E.coli chromosome change depending on the cell growth conditions.

  • Research Products

    (99 results)

All Other

All Publications (99 results)

  • [Publications] Buyukuslu, N.: "Physical mapping of a collection of Mael-generating amber mutations and the functional effect of internal deletions constructed through their manipulation" Genes and Function. 1(2). 119-130 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chugani, S.A.: "Activation of the catBCA promoter:probing the interaction of CatR and RNA polymerase through in vitro transcription." J.Bacteriol.179(7). 2221-2227 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Choy, H.E.: "Repression and activation of prooter-bound RNA polymerase activity by Gal repressor." J.Mol.Biol.272(3). 293-300 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Greiner, D.P.: "Synthesis of the protein cutting reagent iron(S)-1-(p-bromoacetamide-benzym)ethylenediaminetetraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8(1). 44-48 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihama.A.: "Adaptation of gene expression in stationary phase bacteria." Curr.Opn.Genet.Devel.7(5). 582-588 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jeon, Y.H.: "Flexible linker in the RNA polymerase α subunit facilitates the independent motion of the C-terminal activator contact domain." J.Mol.Biol.267. 953-962 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jishage, M.: "Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli strain W3110." J.Bacteriol.179(3). 959-963 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kundu, T.K: "Promoter selectivity of Escherichia coli RNA polymerase O^F holoenzyme involved in transcription of flagellar and chemotaxis genes." J.Bacteriol.179(13). 4264-4269 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusano, S.: "Stimulatory effect of trehalose on the formation and activity of Escherichia coli RNA polymerase EQ^<38> holoenzyme." J.Bacteriol.179(11). 3649-3654 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusano, S.: "Functional interaction of Escherichia coli RNA polymerase with inorganic polyphosphate." Genes to Cells. 2(7). 433-441 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] McHall, S.M.: "DNase I footprinting,DNA bending and in vitro transcription analysis of CIcR and CatR on the clcABD promoter:Evidence of a conserved trascriptional activation mechanism." Mol.Microbiol.24(5). 965-976 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iurakami, K.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact diferent halves of the DNA UP element." Proc.Natl.Acad.Sci.USA. 94(5). 1709-1714 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murakami, K.: "Positioning of two alpha subunits carboxy-terminal domains of RNA polymera at promoters by two transcription factors." Proc.Natl.Acad.Sci.USA.94(21). 11274-11278 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Negre, D.: "DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25(4). 713-718 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozoline, O.N.: "Specific fluorescent labeling of two functional domains in RNA polymeras alpha subunit." PROTEINS:Structure,Function,and Genetics. 30(1). 1-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rajkumari, K: "Evidence for transcription attenuation rendering cryptic O^s-dependent.promoter for the osmotically regulated proU operon of Salmonella typhimun" J.Bacteriol.179(22). 7169-7173 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang, J: "Amino acid residues in the α subunit C-terminal domain of Escherichia coli RNA polymerase involved in interactions with an UP-like sequence and the TyrR protein in the control of transcription from the mtr promoter." J.Bacteriol.179(19). 6187-6191 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chatterji, D.: "The mediator for stringent control,ppGpp,binds to the β-subunit of Escherichia coli RNA polymerase." Genes to Cells. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihama, A.: "Subunits of yeast RNA polymerases in structure and function." Curr.Opn.Microbiol.1(2). 190-196 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jishage, M.: "A stationary phase protein in Escherichia coli with binding activity to the major Q subunit of RNA polymerase." Proc.Natl.Acad.Sci.USA. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake, R.: "The dimeric association of E.coli RNA polymerase alpha subunits,studied by cleavage of Fe-BABE conjugated single-cysteine alpha subunits." Biochemistry. 37(5). 1344-1349 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Owens, J.T.: "Mapping the Sigma-70 Subunit Mapping the Contact Sites on the Core Enzyme Subunits of Escherichia coli RNA polymerase by Site-Specific Cleavage with Sigma-Conjugated Chemical Protease." Proc.Natl.Acad.Sci.USA. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozoline, O.N.: "Topology of the Escherichia coli RNA polymerase surface involved in interaction with promoter upsteam enhancer elements." Eur.J.Biochem.in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozoline, 0.N.: "Specific fluorescent labeling of two functional domains in RNA polymerase alpha subunit." PROTEINS:Structure,Function,and Genetics. 30. 183-192 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihama, A.: "Nucleic Acids & Molecular Biology,Vol.11,Mechanism of Transcription" Springer-Verlag, 327 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihama, A.: "Biomolecular Interactions in DNA and Proteins" Nova Sci.Publ., (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Apriakawam wang, A., Fukuchi, J., Kashiwagi, K., Kakinuma, Y., Ito, E., Ishihama, A.and Igarashi, K.: "Enhancement of cell death due to the inhibition of OmpFsynthesis by RMF deficiency in the double rmf ompC mutant." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ballesteros, M., Kusano, S., Ishihama, A.and Vicente, M.: "The ftsQ1p gearbox promoter of Escherichia coli is a major sigma S-dependent promoter in the ddlB-ftsA refion." Mol.Microbiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chatterji, D., Fujita, N.and Ishihama, A.: "The mediator for stringent control, ppGpp, binds to the beta-subunit of Escherichia coli RNA polymerase." Genes to Cells.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, A., Mizumoto, K.and Ishihama, A.: "A simple enzymatic method for analysis of RNA 5'-terminal structures : Quantitative determination of the 5' termini of influenza virus genome RNAs." Virus Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishiguro, A., Kimura, M., Yasui, K., Iwata, A., Ueda, S.and Ishihama, A.: "Two large subunits of the fission yeast RNA polymerase II provide a platform for the assembly of small subunits." J.Mol.Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A., Kimura, M.and Mitsuzawa, H.: "Subunits of yeast RNA polymerases in structure and function." Curr.Opn.Microbiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A., Zou, C., Kobayashi, M., Kumar, A., Fujita, N., Sakurai, H.and Hayward, R.S.: "Molecular recognition in gene transcription." In : Biomolecular Interactions in DNA and Proteins. (Buckin, V.and Funck, T., eds.). Nova Sci.Publ.(1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jishage, M.and Ishihama, A.: "A stationary phase protein in Escherichia coli with binding activity to the major sigma subunit of RNA polymerase." Proc.Natl.Acad.Sci.USA.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] McFall, S.M., Ishihama, A.and Chakrabatry, A.M.: "Transcriptional acrivation of the clcABD chlorocatechol operon : Interaction between ClcR bound to the promoter proximal site and RNA polymerase." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, R., Murukami, K., Owens, JT., Greiner, DP., Ozoline, O.N., Ishihama, A.and Meares, C.F.: "The dimeric association of E.coli RNA polymerase alpha subunits, studied by cleavage of Fe-BABE conjugated single-cysteine alpha subunits." Biochemistry. 37. 1344-1349 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyao, T., Honda, A., Qu, Z.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe : Subunit-subnit contact sites on Rpbl and Rpb2." Mol.Gen.Genet.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mukherjee, A., Cui, Y., Ma, W.L., Liu, Y., Ishihama, A., Eisenstark, A.and Chatterjee, A.K.: "RpoS (Sigma-S) controls the expression of rmsA,a global regulator of secondary metabolites, Harpin_<ECC> and extracellular proteins in Ervinia carotovora." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, S., Nagasawa, Y., Yagihashi, T.and Ishihama, A.: "The cyclic AMP receptor protein (CRP) gene function is required for expression of alpha (cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect.Immun.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Negre, D., Oudot, C., Prost, J.-F., Murakami, K., Ishihama, A., Cozzone, A.J.and Cortay, J.-C.: "FruR-mediated transcriptional activation at the ppsA promoter of Escherichia coli." J.Mol.Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T., Fujita, N.and Ishihama, A.: "Mapping of subunit-subunit contact sites on the beta subunit of Escherichia coli RNA polymerase."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Owens, J.T., Murakami, K., Chmura, A., Fujita, N., Ishihama, A.and Meares, C.F.: "Mapping lac UV5 DNA sites proximal to conserved regions of sigma-70 in an Escherichia coli RNA polymerase-DNA open promoter complex." Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Owens, J.T., Miyake, R., Murakami, K., Fujita, N., Chmura, A.J., Ishihama, A.and Meares, CF.: "Mapping the Sigma-70 Subunit Mapping the Contact Sites on the Core Enzyme Subunits of Escherichia coli RNA Poymerase by Site-Spesific Cliavage with Sigma-Conjugated Chemical Protease." Proc.Natl.Acad.Sci.USA.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozoline, O.N., Fujita, N., Murakami, K.and Ishihama, A.: "Topoloty of the Escherichia coli RNA polymerase surface involved in interaction with promoter upsteam enhancer elements." Eur.J.Biochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozoline, O.N., Murakami, K., Negishi, T., Fujita, N.and Ishihama, A.: "Specific fluorescent labeling of two functional domains in RNA polymerase alphasubunit." PROTEINS : Structure, Function, and Genetics. 30. 183-192 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryu, S., Fujita, N., Ishihama, A.and Adhya, S.: "GalR-mediated repression and activation of lacUV5 promoter : Different contacts with RNA polymerase." Gene. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sun, W., Hattman, S., Fujita, N.and Ishihama, A.: "Acrivation of bacteriophage Mumom transcription by the C protein does not require specific interaction with the carboxyl terminal region of the alpha and sigma^<70> subunit of Escherichia coli RNA polymerase." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Thomas, M.S., Zou, C., Ishihama, A.and Glass, R.E.: "The effect of a nested set of C-terminal substituted deletions on the function of the alpha subunit of Escherichia coli RNA polymerase." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui, K., ishiguro, A.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe : Subunit-subunit contact sites on Rpb3." Biochemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, P., Yang, J., Ishihama, A.and Pittard, A.J.: "Demonstration that the TyrR prorein and RNA polymerase complex formed at the divergent P3 promoter inhibits the binding of RNA polymerase to the major promoter P1 of the aroP gene of Escherichia coli." J.Bacteriol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asano, Y.and Ishihama, A.: "Identification of two nucleotide-biding sites on teh PB1 subunit of influenza virus RNA polymerase." J.Biochem.122(3). 627-634 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Boucher, P.E., Murakami, K., Ishihama, A.and Stibitz, S.: "The nature of DNA-binding and RNA polymerase interaction of the Bordetella pertusis BvgA transcriptional activator at the fha promoter." J.Bacteriol.179(5). 1755-1763 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Buyukuslu, N., Trigwell, S., Lin, P.P., Ralphs, N.T., Fujita, N., Ishihama, A.and Glass, R.E.: "Physical mapping of a collection of MaeI-generating amber mutations and the functional effect of internal delections constructed through their manipulation." Genes and Function. 1(2). 119-130 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chugani, S.A., Parsek, M.R., Douglas, Hershberger, C.D., Murakami, K., Ishihama, A.and Chakrabarty, A.M.: "Activation of the catBCA promoter : Probing the interaction of CatR and RNA polymerase through in vitro transcription." J.Bacteriol.179(7). 2221-2227 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Choy, H.E., Hanger, R.R., Aki, T., Mahoney, M., Murakami, K., Ishihama, A.and Adhya, S.: "Repression and activation of prooter-bound RNA polymerase activity by Gal repressor." J.Mol.Biol.272(3). 293-300 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Greiner, D.P., Miyake, R., Moran, J.K., Jones, A.D., Negishi, T., Ishihama, A.and Meares, C.F.: "Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidebenzym) ethylenediamineteraacetate and conjucation to cysteine side chains." Bioconjugate Chemistry. 8(1). 44-48 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, A.and Ishihama, A.: "The molecular anatomy of influenza virus RNA polymerase." Biol.Chem.378. 483-488 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A.: "Promoter selectivity control of Escherichia coli RNA polymerase." Nucleic Acids & Molecular Biology, Vol.11, Mechanism of Transcription, Eds.F.Eckstein and D.Lilley. Springer-Verlag, Heidelberg. 53-70 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A.: "Adaptation of gene expression in stationary phase bacteria." Curr.Opn.Genet.Devel.7(5). 582-588 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A., Zou, C., Kobayashi, M., Kumar, A., Fujita, N., Sakurai, H.and Hayward, R.S.: "Molecular recognition in gene transcription." In : Biomolecular Interactions in DNA and Proteins.(Buckin, V.and Funck, T., eds.). Nova Sci.Publ.(1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jeon, Y.H., Yamazaki, T., Otomo, T., Ishihama, A.and Kyogoku, Y.: "Flexible linker in the RNA polymerase alpha subunit facilitates the independent motion of the C-terminal activator contact domain." J.Mol.Bilo.267. 953-962 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jishage, M.and Ishihama, A.: "Variation in RNA prolymerase sigma subunit composition within different stocks of Escherichia coli strain W3110." J.Bacteriol.179(3). 959-963 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang, J.G., Hahn, M.Y., Ishihama, A.and Roe, J.H.: "Identification of sigma factors for growth phase-related promoter selectivity of RNA polymerases from Steptomyces coelicolor A3(2)." Nucleic Acids Res.25. 2566-2573 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, M., Ishiguro, A.and Ishihama, A.: "RNA polymerase II subunits 2,3 and 11 form a core subassembly with DNA binding activity." J.Biol.Chem.272. 25851-25855 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kundu, T.K., Kusano, S.and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA polymerase sigma^F holonenzyme involved in transcription of flagellar and chemotaxis genes." J.Bacteriol.179(13). 4264-4269 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusano, S.and Ishihama, A.: "Stimulatory effect of trehalose on the formation and activity of Escherichia coli RNA polymerase Esigma^<38> holoenzyme." J.Bacteriol.179(11). 3649-3654 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusano, S.and Ishihama, A.: "Functional ionteraction of Escherichia coli RNA polymerase with inorganic polyphosphate." Genes to Cells. 2(7). 433-441 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] McHall, S.M., Klem, T.J., Fujita, N., Ishihama, A.and Chakrabarty, A.M.: "DNase I footprinting, DNA bending and in vitro transcription analysis of ClcR and CatR on the clcABD promoter : Evidence of a conserved transcriptional activation mechanism." Mol.Microbiol.24. 965-976 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami, K., Kimura, M., Owens, J.T., Meares, C.F.and Ishihama, A.: "The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact diferent halves of the DNA UP element." Proc.Natl.Acad.Sci.USA. 94(5). 1709-1714 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami, K., Owens, J.T., Belaeva, T.A., Meares, C.F., Busby, S.J.W.and Ishihama, A.: "Positioning of two alpha subunits carboxy-terminal domains of RNA polymerase at promoters by two transcription factors." Proc.Natl.Acad.Sci.UISA. 94(21). 11274-11278 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Negre, D., Bonod-Bidaud, C., Oudor, C., Prost, J.-F., Kolb, A., Ishihama, A., Cozzone, A.J.and Cortay, J.-C.: "DNA flexbility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter." Nucleic Acids Res.25(4). 713-718 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ozoline, O.N., Masulisi, I.S., Chasovi, V.V.and Ishihama, A.: "Functional topology of the bacterial RNA polymerase." In : Spectroscopy of Biological Molecules : Modern Trends. P.Carmona, R.Navarro and A.Hernanz (eds.). Kluwer Acadenmic Publ., Dordrecht. 163-164 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rajkumari, K., Ishihama, A.and Gowrishankar, J.: "Evidence for transcription attenuation rendering crypric sigma^S-dependent promoter for the osmotically regulated oroU operon of Salmonella typhimurium." J.Bacteriol.179. 7169-7173 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakurai, H.and Ishihama, A.: "Gene organization and sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II." Gene. 196. 165-174 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yang, J., Murakami, K., Camakaris, H., Fujita, N., Ishihama, A.and Pittard, A.J.: "Amino acid residues in the alpha subunit C-terminal domain of Escherichia coli RNA polymerase involed in interactions with and UP-like sequence and the TryR protein in the control of transcription from the mtr promoter." J.Bacteriol.179(19). 6187-6191 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Artsimovitch, I., Murakami, K., Ishihara, A.and Howe, M.M.: "Transcription activation by the bacteriophage Mu Mor protein requires the C-terminal regions of both alpha and sigma^<70> subunists of Escherichia coli RNA polymerase." J.Biol.Chem.271. 32343-32348 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Belyaeva, T., Bown, J., Fujita, N., Ishihama, A.and Busby, S.: "Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region." Nucleic Acids Res.24. 2243-2251 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eichenberger, P., Dethiollaz, S., Fujita, N., Ishihama, A.and Geiselmann, J.: "The influence of the location of the activator binding site on the geometry of a transcriptional activation.complex in Escherichia coli." Bichemistry. 35. 15302-15312 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujita, N., and Ishihama, A.: "Reconstitution of RNA polymerase." Meth.Enzymol. 273, RNA Polymerase and Associated Factors, Part A,Adhya, S., ed.121-130 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gilade, H., Murakami, K., Ishihama, A.and Oppenheim, A.B.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoter of bacteriophage lambda." J.Mol.Biol. 260. 484-491 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A.: "A multi-functional enzyme with RNA polymerase and RNase acrivities : Molecular anatomy of influenza virus RNA polymerase." Biochimie. 78. 1097-1102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imamura, R., Yamanaka, K., Ogura, T., Hiraga, S., Fujita, N., Ishihama, A.and Niki, H.: "Identification of the cdpA gene encoding cyclic 3', 5'-adenosine phosphophate phosphodiesterse in Escherchia coli." J.Biol.Chem.271. 25423-25429 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihama, A., Asano, Y., Honda, A., Toyoda, T., Mizumoto, K.and Nakada, S.: "Structure, function and formation of influenza virus RNA polymerase." In : Options for the Control of Influenza, Eds.L.E.Brown, A.W.Hampson and R.G.Webster. Elsevier Sci.B.V., Amsterdam. 372-380

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jair, K.-W., Fawcett, W.P., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Ambidextrous transcriprion activation by SoxS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19. 307-314 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jair, K.-W., Yu, X., Skarstad, K., Thony, B., Fujita, N., Ishihama A.and Wolf, Jr., R.E.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J.Bacteriol.178. 2507-2513 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jishage, M., Iwata, A., Ueda, S.and Ishihama, A.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli : Intracellular levels of four speoies of sigma subunit under various growth conditions." J.Bacteriol.178. 5447-5451 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawagishi : Kobayashi, M., Yamamoto, M.and Ishihama, A.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol.Gen.Genet.205. 1-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kmura, M.and Ishihama, A.: "Subunit assembly in vivo of Escherichia coli RNA polymerase : Role of the amino-terminal assembly domain of alpha subunit" Genes To Cells. 1. 517-528 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, M., Toyoda, T.and Ishihama, A.: "Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase." Arch.Viorl.141. 525-533 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusano, S., Ding, Q., Fujita, N.and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA Polymerase Esigma^<70> and Esigma^<38> holoenzymes : Effect of DNA supercoiling." J.Biol.Chem.271. 1998-2004 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo, J., Sharif, K.A., Jin, R., Fujita, N., Ishihama, A.and Krakow, J.S.: "Molecular anatomy of the beta' subunit of the E.coli RNA polymerase : Identification of regions involved in polymerase assembly." Genes to Cells. 1. 819-828 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyao, T., Yasui, K., Sakurai, H., Yamagishi, M.and Ishihama, A.: "Molecular assembly of RNA polymerase II from the fission yeast Schizosacharomyces pombe : Subunit-subunit contact network involving Rpb5." Genes to Cells. 1(9). 843-855 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murakami, K., Fujita, N.and Ishihama, A.: "Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element." EMBO J.15. 4358-4367 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rajkumari, K., Kusano, S., Ishihama, A., T.Mizuno and Gowrishankar, J.: "Effects of H-NS and potassium glutamate on sigma^S-andsigma^<70>-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J.Bacteriol.178. 4176-4181 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakurai, H., Miyao, T.and Ishihama, A.: "Subunit composition of RNA polymerase II from the fission yeast Schizosaccharomyces pombe." Gene. 180. 63-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tang, Y., Murakami, K., Ishihama, A.and deHaseth, P.L.: "Upstream interactions at the lambda P_<RM> promoter are sequence-nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRNA promoter." J.Bacteriol.178. 6945-6951 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Adyshev.DM., Kobayashi, M., Iwata, A.and Ishihama, A.: "Molecular assembly of influenza virus RNA polymerase : Identification of the subunit-subunit contact sites on each P protein subunit." J.Gen.Virol.77. 2149-2157 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Adyshex.DM., Kobayashi, M., Iwata, A.and Ueda, S.and Ishihama, A.: "The subunit-subunit contact sites of the influenza virus RNA polymerase." Oprions for Control of Influenza, Eds.L.E.Brown, A.W.Hampson and R.G.Webster. Elsevier Sci.B.V., Amsterdam.419-426 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoda, T., Kobayashi, M., Nakada, S and Ishihama, A.: "Molecular dissection of influenza virus RNA polymerase : PB1 subunit alone is able to catalyze RNA synthesis." Virus Genes. 12. 155-161 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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