1996 Fiscal Year Final Research Report Summary
Selection of male and female embryos before implantation
| Project/Area Number |
08458238
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
OKABE Masaru Osaka University, Research Institute for Microbial Diseases, Associate Professor, 微生物病研究所, 助教授 (30089875)
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| Project Period (FY) |
1996
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| Keywords | GFP / transgenic mouse / X chromosome / Y chromosome / X inactivation |
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| Research Abstract |
The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. All of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments.
In order to separate the X and Y baring embryo, we have produced many lines of transgenic mouse. Among then, we had succeeded to produce three lines of X-linked GFP and one line of Y-linked GFP.
These mice would serve as a study in separation of male and female embryos before implantation. It would also serve as a powerful tool to investigate the mechanism of X inactivation.
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Research Products
(9 results)
