1996 Fiscal Year Annual Research Report

神経細胞死抑制因子としてのPACAP

Research Project

Project/Area Number	08458249
Research Category	Grant-in-Aid for Scientific Research (B)
Research Institution	Showa University
Principal Investigator	塩田清二昭和大学, 医学部, 講師 (80102375)
Co-Investigator(Kenkyū-buntansha)	水島秀勝昭和大学, 医学部, 講師中条茂男昭和大学, 薬学部, 助教授 (50119236)
Keywords	PACAP / 神経細胞死 / 海馬 / 免疫組織化学 / 遺伝子組織化学
Research Abstract	我々はPACAP(Pituitary Adenylate Cyclase-Activaing Polypeptide)による神経細胞死抑制のメカニズムを解明すべく、実験的脳虚血ラットを用いて、免疫組織化学法および遺伝子組織化学法を用いて観察を行った。その結果、脳虚血に伴い、海馬CA1領域の神経細胞と神経膠細胞にPACAPレセプターの発現が顕著にみられた(Uchida et al.1996)。PACAPの脳室内投与により神経細胞死が有意に抑制されたことから、PACAPはおそらく神経細胞の細胞内のcAMPを上昇させ、何らかのシグナル伝達経路を介して神経細胞死を防ぐ可能性が示唆された(Banks et al.1996;水島ら1996)。我々は、PACAPが視床下部神経分泌ニューロンの細胞内カルシウムを顕著に増加させ、しかもcAMP-protein kinase Aを介するシグナル伝達機構の関与をみとめている(Shioda et al.1996)。PACAPは神経細胞死の抑制効果をもち、その抑制効果は神経膠細胞を介して行われる可能性のあることが培養下の実験結果から示唆されており、今回のin vivoの実験観察結果はそれを支持するものと考えられる。PACAPは神経膠細胞に作用し種々のサイトカインの合成や分泌を促進し、それが神経細胞死を抑制する可能性、あるいは神経膠細胞自身の分裂・増殖を誘起する可能性もあると考えられることから、さらにPACAPによる神経細胞死抑制機構の詳細を解明するために平成9年度も継続して研究する予定である。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Mizushima,H.: "Time-dependent changes of rasoactive substances in the rat anygdaloid complex after transient cerebral ischemia." Regulatory Peptides. 64. 127 (1996)
[Publications] Shioda,S.: "Pituitary adenylate cyclase-activating polypeptide : a key player in male reproduction?" Amalus of Endocrinology. 57. 57 (1996)
[Publications] Shioda,S.: "Distribution of PACAP receptor mRNA and PACAP receptor-like inmunoreactivity in the rat brain." 解剖学雑誌. 71. 364 (1996)
[Publications] 塩田,清二: "視床下部神経分泌ニューロンのPACAP性神経支配とそのシグナル伝達機構" 日本臨床電子顕微鏡学会誌. 29. S81 (1996)
[Publications] Homma,H.: "Effect of focal cerebral ischemia on nitricoxide synthase expression in rats." Medical Electron Microscopy. (印刷中). (1997)
[Publications] Shioda,S.: "Pituitary adenylate cyclase-activating polypeptide : a key player in male reproduction?" Ann,NY Acad.Sci.(in press). (1997)
[Publications] Seki,T.: "Localization of PACAP receptor and its transcript in the rat retina determined by immunocytochemistry and insitu hybridization." Acta Histochemica et Cytochemica. 29(suppl.). 882-883 (1996)
[Publications] Shioda,S.: "Cellular localization and gene expression of alpha 4 neulonal mncatinic receptors in the rat hypothalamus." Acta Histochemica et Cytochemica. 29(suppl.). 1018-1019 (1996)
[Publications] Mizushima,H.: "Morphological changes of c-Fos-like immunoreactivity in rat cerebral cortex after brain ischemia and reperfusion." Acta Histochemica et Cytochemica. 29(suppl.). 1026-1027 (1996)
[Publications] Homma,H.: "Time-dependent changes of nitricoxide Synthase immunoreactivity in rat cerebral cortex following focal ischemia." Acta Histochemica et Cytochemica. 29(suppl.). 1053-1054 (1996)
[Publications] Shioda,S.: "Direct projections from contecholaminergic neurons in the caudal ventrolateral medulla to vasopressin-containing neurons." Neuroscience Letters. 221. 45-48 (1996)
[Publications] Shioda,S.: "Cellular localization and functional significance of PACAP anlits receptors in rat hypothalamus." Regulatory Peptides. 64. 174 (1996)
[Publications] Uchida,D.: "Cytoprotective action of pituitry Adenylate Cyclase Activating Polypeptide in ischemia-induced neuronal cell death in the rat hipeocampus." Brain Research. 736. 280-286 (1996)
[Publications] Banks,W.A.: "Transport of pituitary adenylate cyclase-activating polypeptides across the blood-bimin barrier and prevention of ischemia-induced cell death." Ann.NY Acad.Sci.805. 270-279 (1996)
[Publications] 水島秀勝: "PACAPによる遅発性神経細胞死抑制効果について-分子神経科学的研究-" 神経化学. 35. 310-311 (1996)
[Publications] Shioda,S.: "PACAP and its type I receptors in the rat hypothalamcs ; neuroendocrine interaction." Ann.NY Acad.Sci.805. 670-676 (1996)
[Publications] Shioda,S.: "Localization of PACAP and its type I receptor in the rat ovary : immunohistochemistry and in situ hybridization." Ann.NY Acad.Sci.805. 677-683 (1996)
[Publications] Yada,T.: "Current status of PACAP as a regulator of insulin secration in pancreatic islets." Ann.NY Acad.Sci.805. 329-342 (1996)

1996 Fiscal Year Annual Research Report

神経細胞死抑制因子としてのPACAP

Principal Investigator

塩田 清二 昭和大学, 医学部, 講師 (80102375)

Research Products

[Publications] Mizushima,H.: "Time-dependent changes of rasoactive substances in the rat anygdaloid complex after transient cerebral ischemia." Regulatory Peptides. 64. 127 (1996)

[Publications] Shioda,S.: "Pituitary adenylate cyclase-activating polypeptide : a key player in male reproduction?" Amalus of Endocrinology. 57. 57 (1996)

[Publications] Shioda,S.: "Distribution of PACAP receptor mRNA and PACAP receptor-like inmunoreactivity in the rat brain." 解剖学雑誌. 71. 364 (1996)

[Publications] 塩田,清二: "視床下部神経分泌ニューロンのPACAP性神経支配とそのシグナル伝達機構" 日本臨床電子顕微鏡学会誌. 29. S81 (1996)

[Publications] Homma,H.: "Effect of focal cerebral ischemia on nitricoxide synthase expression in rats." Medical Electron Microscopy. (印刷中). (1997)

[Publications] Shioda,S.: "Pituitary adenylate cyclase-activating polypeptide : a key player in male reproduction?" Ann,NY Acad.Sci.(in press). (1997)

[Publications] Seki,T.: "Localization of PACAP receptor and its transcript in the rat retina determined by immunocytochemistry and insitu hybridization." Acta Histochemica et Cytochemica. 29(suppl.). 882-883 (1996)

[Publications] Shioda,S.: "Cellular localization and gene expression of alpha 4 neulonal mncatinic receptors in the rat hypothalamus." Acta Histochemica et Cytochemica. 29(suppl.). 1018-1019 (1996)

[Publications] Mizushima,H.: "Morphological changes of c-Fos-like immunoreactivity in rat cerebral cortex after brain ischemia and reperfusion." Acta Histochemica et Cytochemica. 29(suppl.). 1026-1027 (1996)

[Publications] Homma,H.: "Time-dependent changes of nitricoxide Synthase immunoreactivity in rat cerebral cortex following focal ischemia." Acta Histochemica et Cytochemica. 29(suppl.). 1053-1054 (1996)

[Publications] Shioda,S.: "Direct projections from contecholaminergic neurons in the caudal ventrolateral medulla to vasopressin-containing neurons." Neuroscience Letters. 221. 45-48 (1996)

[Publications] Shioda,S.: "Cellular localization and functional significance of PACAP anlits receptors in rat hypothalamus." Regulatory Peptides. 64. 174 (1996)

[Publications] Uchida,D.: "Cytoprotective action of pituitry Adenylate Cyclase Activating Polypeptide in ischemia-induced neuronal cell death in the rat hipeocampus." Brain Research. 736. 280-286 (1996)

[Publications] Banks,W.A.: "Transport of pituitary adenylate cyclase-activating polypeptides across the blood-bimin barrier and prevention of ischemia-induced cell death." Ann.NY Acad.Sci.805. 270-279 (1996)

[Publications] 水島秀勝: "PACAPによる遅発性神経細胞死抑制効果について-分子神経科学的研究-" 神経化学. 35. 310-311 (1996)

[Publications] Shioda,S.: "PACAP and its type I receptors in the rat hypothalamcs ; neuroendocrine interaction." Ann.NY Acad.Sci.805. 670-676 (1996)

[Publications] Shioda,S.: "Localization of PACAP and its type I receptor in the rat ovary : immunohistochemistry and in situ hybridization." Ann.NY Acad.Sci.805. 677-683 (1996)

[Publications] Yada,T.: "Current status of PACAP as a regulator of insulin secration in pancreatic islets." Ann.NY Acad.Sci.805. 329-342 (1996)

塩田清二昭和大学, 医学部, 講師 (80102375)