1997 Fiscal Year Final Research Report Summary
Structural and functional studies on the carbohydrates of the adhesion glycoproteins in nervous system.
| Project/Area Number |
08458255
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | SAITAMA MEDICAL SCHOOL |
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Principal Investigator |
KITAMURA Kunio Saitama Medical School, Physiology, Associate Professor, 医学部, 助教授 (70049857)
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| Project Period (FY) |
1996 – 1997
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| Keywords | peripheral nerve myelin / adhesion proteins / carbohydrate structure / sulfated glucurenic acid / HNK-1 / biosensor |
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| Research Abstract |
We have analyzed the oligosaccharides of the glycoproteins (PO and PAS-II/PMP-22) in peripheral nerve myelin from various mammalian species. The oligosaccharide moieties of both proteins contain sulfated glucuronic acid as a common constituent, suggesting that the sulfated glucuronic acid may be a key molecule in the adhesion functions of the glycoproteins. Since the PO protein is known to play a role in adhesion of myelin membranes by a homophilic manner, carbohydrate-carbohydrate interaction was analyzed by biosensor apparatus. However any interaction among oligosaccharides could not be observed. Then we have analyzed the interaction between oligosaccarides and polypeptides from PO protein by an affinity column. One peptide fragment which interacted specifically with sulfated glucuronic acid was obtained. By the sequence analysis of the peptide revealed that the peptide has a significant homology to the laminin G2 domain, which is known to be an essential site of sulfated glucuronic acid binding property of laminin. Therefore, the sulfated glucuronic acid is the requisite molecule for the homophilic adhesion property of the PO protein and eventually for myelination.
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[Publications] Ogawa, S., Iahibashi, Y., Sakamoto, Y., Kitamura, K., Kubo, M., Sakai, T.& Inoue, K.: "The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments." Biochem.Biophys.Res.Commun.234・3. 712-718 (1997)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ogawa, S., Ishibashi, Y., Sakamoto, Y., Kitamura, K., Kubo, M., Sakai, T.and Inoue, K.: "The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments." Biochem.Biophys.Res.Commun.234 (3). 712-718 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Akiyama, Y., Suzuki, T., Tanaka, M., Imai, F., Asaoka, T., Sakamoto, Y.and Kitamura, K.: "A case of eosinophilia-myalgia syndrome complicated with recurrent parotitis." Allergy. 46 (12) (in Japanese). 1273-1276 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshimura, K., Negishi, T., Kaneko, A., Sakamoto, Y., Kitamura, K., Hosokawa, T., Hamaguchi, K., and Nomura, M.: "Monoclonal antibodies specific to the integral membrane protein PO of bovine peripheral nerve myelin." Neurosci.Res.25 (1). 41-49 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Kitamura, K., Hosokawa, T., Sakamoto, Y., Terada, S., Yoshimura, K.and Nomura, M.: "Screening of autoantibodies in the serum from neuropathy patients by biosensor." Shinkeikagaku. 35 (3)(in Japanese). 262-263 (1996)
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「研究成果報告書概要(欧文)」より
