1997 Fiscal Year Final Research Report Summary
DNA Biosensors for Medical Diagnosis
| Project/Area Number |
08458290
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MAEDA Mizuo Kyushu University Materials Physics and Chemistry Professor, 工学部, 教授 (10165657)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Koji Kyushu University Chemicals Systems Engineering Associate Professor, 工学部, 助教授 (10180324)
KATAYAMA Yoshiki Kyushu University Materials Physics and Chemistry Associate Professor, 工学部, 助教授 (70284528)
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| Project Period (FY) |
1996 – 1997
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| Keywords | DNA biosensor / anti-DNA antibody / gene sensor / drug / oligonucleotide / voltammetry / SLE / electrochemical sensor |
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| Research Abstract |
Most of biosensors rely on proteins such as enzymes and antibodies, while DNA is also an important host molecule in biological affinity reactions. DNA-immobilized electrode may be a usuful tool for medical diagnosis. We immobilized DNA double strands on a Au electrode via chemisorption. Cyclic voltammograms of ferrocyanide were taken with the DNA-modified electrode. When adding DNA-binding molecules, the CV peak currents increased with increasing concentration of them. The peak current showed a sigmoidal relationship with the logarithmic concentration of the added compound. The sensor comprising DNA was confirmed to be a useful tool for evaluation of the interaction between DNA and DNA-binding molecules. In addition, this DNA-modified electrode was found to respond to anti-DNA antibody which specifically binds to double helical DNA.Anti-DNA antibodies has been used as marker molecule of Systemic Lupus Erythematossus (SLE). We also developed a novel electrochemical technique for gene detection in which the sandwich-type complex from oligodeoxynucleotide (ODN) immobilized on Au electrode, target DNA,and the ferrocenyl ODN is formed to give a response. Differential pulse voltammograms for the modified electrode showed a significant anodic peak due to the oxidation of ferrocene moiety of the probe DNA,when the electrode was treated with the target DNA.On the other hand, only a slight peak was observed for the modified electrode which was treated similarly with the mismatch control. These results indicate that the target was recognized by means of complementary base-pairing with the ODN immobilized on thw electrode.
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