1998 Fiscal Year Final Research Report Summary
Characterization of a signaling molecule assembly region within the cell membrane
| Project/Area Number |
08459016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
MAEKAWA Shohei Kyoto Institute of Technology, Textile Science Associate Professor, 繊維学部, 助教授 (40173695)
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| Project Period (FY) |
1996 – 1998
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| Keywords | neuronal raft / cytoskeleton / GP1-anchored proteins / paralemin / NAP-22 / cholesterol / calmodulin / GAP-43 |
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| Research Abstract |
Characterization of a signaling molecule assembly region(raft) within the cell membrane in the brain tissue resulted in the following findings :
1. Major components were identified as, tubulin, actin, NAP-22, GAP-43, and several trimeric G proteins, using western blotting and partial amoni acid sequencing analysis.
2. Concentration of several GPI-anchoed proteins, such as F3/contactin, T-cadherin, OBCAM, and neurotrimin was found. In addition, a novel GPI-anchored protein was idenfied and cloned. This protein, named kilon, showed a wide distribution in the brain cortex.
3. Production of monoclonal antobodies using raft as an immunogen resulted in the identification of a protein focalized in raft. This protein was identified as paralemin from its amino acid sequence.
4. Lipid analysis of raft showed the enrichment of phosphatidylcholine and cholesterol in this fraction. Extraction of cholesterol using methy-cyclodextrin caused specific solubilization of NAP-22. Further studies showed the binding of NAP-22 to a cholestrol containing liposome.
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[Publications] Maekawa, S., Kumanogoh, H., Funatsu, N., Takei, N., Inoue, K., Endo, Y., Hamada, K., and Sokawa, Y.: "Identification of NAP-22 and GAP-43 (neuromodulin) as major protein components in a Triton insoluble low density fraction of rat brain." Biochimica Biophysica Acta. 1323. 1-5 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Funatsu, N., Miyata, S., Kumanogoh, H., Shigeta, M., Hamada, K., Endo, Y., Sokawa, Y., and Maekawa, S.: "Characterization of a novel rat brain GP1-anchored protein (kilon), a member of the lgLON Cell adhesion molecule family" J.Biological Chemistry. (in press). (1999)
Description
「研究成果報告書概要(欧文)」より
