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1997 Fiscal Year Final Research Report Summary

Study of genetic variability in the wildlife (Hokkaido brown bear)

Research Project

Project/Area Number 08556053
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 生物資源科学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KANAGAWA Hiroshi  Hokkaido Univ., Graduate School of Vet.Med., Pro., 大学院・獣医学研究科, 教授 (00111162)

Co-Investigator(Kenkyū-buntansha) YAMANAKA Masami  Town of Shari, Dvision of Environmental Conservation, Researcher, 研究職員
MANO Tsutomu  Hokkaido Institute of Environmental Sciences, Researcher, 研究職員
HISHINUMA Mitsugu  Tottori Univ., Fac.of Agri., Associate. Pro., 農学部, 助教授 (30183578)
Project Period (FY) 1996 – 1997
KeywordsHokkaido brown bear / Semen collection / Cryopreservation of spermatozoa / Genetic variability / Microsatellite
Research Abstract

1.Semen collection and cryopreservation of spermatozoa in Hokkaido brown bear
(1) Semen collection was succes using electroejaculation in Hokkaido brown bear. The obtained ejaculates provided baseline data of bear semen, such as ejaculate volume. sperm concentration, sperm motility, and sperm abnormality rate.
(2) The study of freezing technique for bear semen using Tris-buffered egg yolk extender provided the optimal condition, such as final glycerol concentration, equilibration period, and cooling rate. The characteristics of post-thaw semen showed that the mean sperm motility and the mean percentage of live spermatozoa of post-thaw semen were 36.0% and 65.0%, respectively.
(3) Annual changes in serum testosterone concentration, serum FSH concentration and seminal traits were investigated. The increase in serum testosterone concentration was followed by the increases of sperm concentration. The pattern of the annual change of serum FSH concentration was similar to that in testis volume.
2.Genetic variability of Hokkaido brown bear
(1) About 300 liver samples for DNA research were collected at the Hokkaido Institute of Environmental Science.
(2) The effort of searching microsatellite region is maintained, though any individually variable microsatellite region has not been detected. Eight previously developed primer sets for microsatellites of another bear species were applied to 54 captive Hokkaido brown bears. As the result, 4 to 10 (6.5+1.9) alleles were detected, and the average heterozygosity (gene diversity) was 0.67+0.12. These values are similar to those of another bear species and may suggest that the applied primer sets were thought to be effective for evaluating the genetic variability of Hokkaido brown bears. At present, analysis of DNA samples extracted from wild bears is doing with those primer sets.
(3) The same research as Hokkaido brown bear with those 8 primer sets are performing about the Japanese black bear.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Akiko Ishikawa: "Attempts to collect and freeze the semen of the Hokkaido brown bear." Proceedings of satellite symposium in the 8th AAAP;conservation biology for human and animal living. 1996. 27-28 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 間野 勉: "生存個体の追跡によるクマ類の個体群解析の現状と課題" 哺乳類科学. 35(2). 180-182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 釣賀 一二三: "北海道のヒグマ(Ursus arctos yesoensis)におけるタンパク多型の欠如" 哺乳類科学. 36(1). 59-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石川 明子: "エゾヒグマの精液採取および凍結保存" 繁殖技術. 17(2). 23-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsutomu Mano: "Harvesthistory of brown bears in the Oshima Peninsula,Hokkaido,Japan." International Conference of Bear Reserch and Management. 10(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akiko Ishikawa: "Attempts to collect and freeze the semen of the Hokkaido brown bear." Proceedings of satellite symposium in the 8th AAAP,conservation biology for human and animal living. 27-28 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsutomu Mano: "Analysis of bear populations in Japan with capture-recaputure and telemetry data." Honyurui Kagaku (Mammalian Science). Vol.35, No.2. 180-182 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hifumi Tsuruga: "Lack of protein polymorphism in Hokkaido brown bears (Ursus arctos yesoensis)" Honyurui Kagaku (Mammalian Science). Vol.36, No.1. 59-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akiko Ishikawa: "Collection and cryopreservation of semen of the Hokkaido brown bear (Ursus arctos yesoensis)" Hanshoku Gijutsu (Technic for Animal Reproduction). Vol.17, No.2. 23-25 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsutomu Mano: "Harvesthistory of brown bears in the Oshima Peninsula, Hokkaido, Japan." International Conference of Bear Reserch and Management. Vol.10, (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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