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1998 Fiscal Year Final Research Report Summary

Development of full-automatic in situ hybridization system for Diagnosis

Research Project

Project/Area Number 08557020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOMURA Shintaro  Osaka University Medical School, Associate professor, 医学部, 助教授 (80159087)

Co-Investigator(Kenkyū-buntansha) HOJOU Shoji  Sakura Instrument, Pathological Research laboratory, Head, 開発本部・病理学研究室, 室長
KITAMURA Yukihiko  Osaka University Medical School, Professor, 医学部, 教授 (70028520)
Project Period (FY) 1996 – 1998
KeywordsDigoxigeuin / Virus / DNA / mRNA / Pathological Diagnois / in situ hybrildization / Hybridization / Pathological Diagnosis
Research Abstract

We first tested the availability of commercially available "Full automatic histological staining system" and "Full automatic immunostaining system for in situ hybridization system. The combination of both systems did work considarably well in not only to detect viral DNA but mRNAs. However, we recognized that some parts of the instrument need to much more heat resistant for long term use. So we changed some of the parts to the goods resistant to high temperature. Next we investigated the TSA, the amplifier to detect signal. We confirmed the detection limit of the signal raised to approximately 50 times than usual system. This amplifier would be useful for obtaining stable result of in situ hybridization. Finally we tested if we can detect gene amplified product of oncogenes such as ras and sam-1 from sample obtained operation. As a result, we detected amplified sam-1 gene at the efficiency of 30% in the sample. This value was consistent from that obtained by Southern blotting. However, it was difficult to detect amplified gene from pathological samples which were already fixed by formaline. This may due to the regradation of DNA during fixiation. We still need to search optical condition to detect nucleic acid from pathological samples.

Research Products

(12 results)

All Other

All Publications

  • [Publications] M.Sato, S.Nomura, et al.: "Transcriptional regulation of o*leopont*** gene in vivo by P*BP**A/CBFA 1 and T*** 1 in the skeletal tissues"Oncogene. 17. 1517-1525 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Ohshima, S.Nomura, et al.: "IL-6 plays a key role in the development of antigen-induced arthrili*sis"Proc. Natol, Acad. Sci. U.S.A. 95. 8222-8226 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Suzuki, S.Nomura, et al.: "Localization of mRNA for trkB isoforms and p75 in the rat retin*l ganglion cells"J. of Neurosci. Res.. 54. 27-37 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Kosaka, S.Nomura, et al.: "Differential localization and expression of α and * isoenzymes of protein kinase C in the rat retina"J. of Neurosci. Res.. 54. 655-663 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Suoda, S.Nomura, et al.: "Maturational disturbance of chondrocytes in CBfa-1,-deficient **i*o"Dev. Dynamics. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Terai, S.Nomura, et al.: "The role of osteopo*tin in the bone re**o*leli*y caused by mechanical stress"J. of Bone and Mineral Res.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Sato, S. Nomura et al.: "Transcriptional regulation of osteopontin gene in vivo by PEBP2A/CBFA1 and Ets-1 in the skeletal tissues"Oncogene. 17. 1517-1525 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Ohshima, S. Nomura et al.: "IL-6 plays a key role in the development of antigen-induced arthritis (AIA)"Proc. Natl. Acad. Sci.. 95. 8222-8226 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Suzuki, S. Nomura et al.: "Localization of mRNA for TrkB isoforms and p75 in the rat retinal ganglion cells"J. of Neurosci Res.. 54. 27-37 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Kosaka, S. Nomura. Et al.: "Differential localization and expression of A and B isoenzymes of protein kinase C in the rat retina"J. of Neurosci. Res.. 54. 655-663 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Inada, S. Nomura et al.: "Maturational disturbance of chondrocyte in cbfa1-deficient mice"Dev. Dynamics.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Terai, S. Nomura, et al.: "The role of osteopontin in the bone remodellijng caused by mechanical stress"J. Bone and Mineral. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-22  

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