1997 Fiscal Year Final Research Report Summary
Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application
| Project/Area Number |
08557059
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
内分泌・代謝学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SAITO Yoshihiko Kyoto Univ., Grad.Sc.of Med., Assistant Professor, 医学研究科, 助手 (30250260)
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| Co-Investigator(Kenkyū-buntansha) |
NIYAMOTO Tsumoru Biol.-Pharmacological.Res.Lab., JAPAN TOBACCO,INC., Central Pharmaceutical.Res.I, 医薬総合研究所・生物研究所, 所長
NISHIMURA Kazunobu Kyoto Univ., Grad.Sc.of Med., Associate Professor, 医学研究科, 講師 (70252450)
NAKAO Kazuwa Kyoto Univ., Grad.Sc.of Med., Professor, 医学研究科, 教授 (00172263)
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| Project Period (FY) |
1996 – 1997
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| Keywords | omentum / migration factor of USMC / phosphadilglycerol |
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| Research Abstract |
Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.
In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.
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Research Products
(12 results)
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[Publications] Harada M,Itoh H,Nakagawa O,Ogawa Y,Miyamoto Y,Kuwahara K,Ogawa E,Igaki T,Yamashita J,Masuda I,Yoshimasa T,Tanaka I,Saito Y,Nakao K.: "Significance of Ventricular Myocytes and Nonmyocytes Interaction During Cardiocyte Hytertrophy. -Evidence for Endothelin-1 as a Paracrine Hypertrophic Factor from Cardiac Nonmyocytes-" Circulation. 96. 3737-3744 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Miyamoto Y,Saito Y,Kajiyama N,Yoshimura M,Shimasaki Y,Nakayama M,Kamitani S,Harada M,Ishikawa M,Kuwahara K,Ogawa E,Hamanaka I,Takahashi N,Kaneshige T,Teraoka H,Akamizu T,Azuma N,Yoshimasa Y,Yoshimasa T,Itoh H,Masuda I,Yasue H,Nakao K.: "Endothelial Nitric Ozide Synthase Gene is Positively Associated With Essential Hypertension." Hypertension. (in press). (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Suda M,Ogawa Y,Tanaka K,Tamura N,Yasoda A,Takigawa T,Uehira M,Nishimoto H,Itoh H,Saito Y,Shiota K,Nakao K.: "Skeletal overgrowth in transgenic mice taht overexpress brain natriuretic peptide." Proc.Natl.Acad.Sci.USA. (in press). (1998)
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[Publications] Shimasaki Y,Hirofumi Y,Michihiro Y,Nakayama M,Kugiyama K,Ogawa H,Harada E,Masuda T,Koyama W,Saito Y,Miyamoto Y,Ogawa Y,Nakao K.: "Association of the Missense Glu 298 Asp Mutation of the Endothelial Nitric Oxide Synthase Gene With Myocardial Infarction." J.Am.Coll.Cardiol.(in press). (1998)
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「研究成果報告書概要(欧文)」より
