1997 Fiscal Year Annual Research Report

激症肝不全治療に関する研究:部分生体肝を用いた補助的一時的同所性移植の実験的検討

Research Project

Project/Area Number	08557078
Research Institution	Showa University
Principal Investigator	中野浩昭和大学, 医学部, 講師 (10241035)
Co-Investigator(Kenkyū-buntansha)	熊田馨昭和大学, 医学部, 教授 (00025602) 山口真彦昭和大学, 医学部, 講師 (00266149)
Keywords	補助的一時的部分同所性肝移植 / N-acetylcysteine / α-glutathione-S-transferase / mitochondrial oxidative stress / neutrophil oxidative stress / ヒアルロン酸
Research Abstract	補助的一時的部分同所性肝移植(Auxiliary Temporal Partial Orthotopic Liver Transplantation(ATPOLT))の動物実験を計画するにあたり,まず,総胆管結紮1週間後に肝左葉切除を行いことによりfulminant hepatic failureモデルを作成し,3日後にdonor犬より肝左葉を生体肝移植の手技を用いて摘出し,これを移植するモデルを作成して検討した.[方法]雄性ビ-グル犬の総胆管結紮により,閉塞性黄疸モデルを作成し,1週間後に肝左葉切除を施行した.このモデルについて,移植後にN-acetylcysteine(NAC)を投与する群と,非投与群(5%glucose持続投与)とを作成した.測定項目として,好中球の活性酸素産生能をflow cytometryで測定した.また,肝ミトコンドリアoxidative stressは,肝ミトコンドリア分画の分離後,HPLCにより肝ミトコンドリアの還元型グルタチオン(GSH)と酸化型グルタチオン(GSSG)を同時測定して,GSSG/GSH値で検討した.肝ジヌソイド内皮細胞機能は,ヒアルロン酸を測定した.肝細胞障害は血中のα-glutathione-S-transferase(αGST)濃度で検討した.[結果]現在までに,両群とも3例の手術が成功した.24時間後のデータとして,好中球oxidative stress,ミトコンドリアoxidative stressは,無治療群で高値であった.ヒアルロン酸値,αGST値も無治療群で高値であった.[結論]Free radical scavengerであるNACを投与すると,mitochondrial oxidative stressやneutrophil oxidative stressが抑制され,肝細胞障害,肝ジヌソイド内皮機能が保持されることが示された.

Research Products
(5 results)

All Other

All Publications (5 results)

[Publications] Nakano H,et al.: "The effects of N-acetylcysteine and anti-intercellular adhesion molecule-1 monoclonal antibody against ischemia-reperfusion injury of the rat steatotic liver produced by choline-methionine deficient diet." Hepatology. 26. 670-678 (1997)
[Publications] Nakano H,et al.: "Prediction of postoperative decompensated liver function by technetium 99m galactosyl human serum albumin liver scintigraphy in patients with hepatocellular carcinoma complicated with chronic liver diseases." British Journal of Surgery. 84. 793-796 (1997)
[Publications] Nakano H,et al.: "Augmentation of mitochondrial glutathione by S-adenosyl-L-methionine in ischemia-reperfusion injury of the rat steatotic liver induced by choline-methionine deficient diet." European Surgical Research. 30. 34-42 (1998)
[Publications] Nakano H,et al.: "Predicting Survival and Postoperative Complications with Tc-GSA Liver Scintigraphy in Hepatocellular Carcinoma." Hepato-gastroenterology. (in press).
[Publications] Nakano H,et al.: "Allopurinol reduced hepatic ischemia-reperfusion injury exacerbated by inhalation of high-concentration oxygen in rats." European Surgical Research. 29. 429-437 (1997)

1997 Fiscal Year Annual Research Report

激症肝不全治療に関する研究:部分生体肝を用いた補助的一時的同所性移植の実験的検討

Principal Investigator

中野 浩 昭和大学, 医学部, 講師 (10241035)

Research Products

[Publications] Nakano H,et al.: "The effects of N-acetylcysteine and anti-intercellular adhesion molecule-1 monoclonal antibody against ischemia-reperfusion injury of the rat steatotic liver produced by choline-methionine deficient diet." Hepatology. 26. 670-678 (1997)

[Publications] Nakano H,et al.: "Prediction of postoperative decompensated liver function by technetium 99m galactosyl human serum albumin liver scintigraphy in patients with hepatocellular carcinoma complicated with chronic liver diseases." British Journal of Surgery. 84. 793-796 (1997)

[Publications] Nakano H,et al.: "Augmentation of mitochondrial glutathione by S-adenosyl-L-methionine in ischemia-reperfusion injury of the rat steatotic liver induced by choline-methionine deficient diet." European Surgical Research. 30. 34-42 (1998)

[Publications] Nakano H,et al.: "Predicting Survival and Postoperative Complications with Tc-GSA Liver Scintigraphy in Hepatocellular Carcinoma." Hepato-gastroenterology. (in press).

[Publications] Nakano H,et al.: "Allopurinol reduced hepatic ischemia-reperfusion injury exacerbated by inhalation of high-concentration oxygen in rats." European Surgical Research. 29. 429-437 (1997)

中野浩昭和大学, 医学部, 講師 (10241035)