1997 Fiscal Year Annual Research Report

セメント質と歯根膜をともなったハイブリッド型人工歯根の開発

Research Project

Project/Area Number	08557096
Research Institution	Hokkaido University
Principal Investigator	久保木芳徳北海道大学, 歯学部, 教授 (00014001)
Co-Investigator(Kenkyū-buntansha)	滝田裕子北海道大学, 歯学部, 教務職員 (30125330) 藤沢隆一北海道大学, 歯学部, 助手 (40190029) 水野守道北海道大学, 歯学部, 助手 (10125354) 大畑昇北海道大学, 歯学部, 教授 (60002185)
Keywords	人工歯根 / セメント質 / 歯根膜 / BMP(骨形成タンパク質) / 細胞支持体
Research Abstract	【研究目的】現在、歯を喪失した場合の修復法の一つに骨癒着型の人工歯根が広く用いられているが、このシステムは必須の緩衝装置である歯根膜を欠くため早晩、歯槽骨を損傷し、多くは数年で脱落あるいは機能不調に陥る。この点を改善するため自然の歯根膜とセメント質を再生する原理と技術を確立し、さらに歯根膜とセメント質付きの人工歯根を創製する。【方法】我々は昨年度までに骨形成サイトカインである骨形成蛋白質(BMP)の抽出方法を確立し、強力な活性BMP(BMPカクテル)を大量に準備した。次に我々が開発検討した8種類以上の細胞支持体のなかでコラーゲン線維膜とBMPと組み合わせた複合体を組織工学的素材として用いた。ビ-グル犬の歯を抜去し、再植する時に歯根膜を、ベルト状(Belt type pefect)と、根尖側2分の1(Apical-half type defect)という2種の規格にしたがって部分削除し、その欠損部にBMP複合コラーゲン線維膜を巻き付けて再植し、経時的に定量的に観察した。削除しない再植例(正常再値例)と削除したがBMPを投与しない例と、2種類の対照をおいた。【結果と今後の展望】 6週目のapical half型組織学的所見では、残存部と欠損部の境界から有細胞セメント質と歯根膜が再生するのが確認された。しかし、根尖部に近づくにしたがって歯根膜形成よりも骨癒着(Ankylosis)が多くなった。これに対しbelt-typeでは良好な歯根膜とセメント質の再生がみられた。新生セメント質は、切削された象牙質の表面に僅かに露出したコラーゲン線維を足場にして、先ず線維性の層状組織をつくり、次第に石灰化してセメント質を作る。この線維性組織層中のコラーゲン線維が、連続して層外に延び出し、大略、垂直性に走行し歯槽骨にまで達している。今後担体としてのコラーゲン線維膜の幾何学的溝造を改善することによりさらに広範囲の歯根膜とセメント質が再生できる見込みである。

Research Products
(27 results)

All Other

All Publications (27 results)

[Publications] 久保木芳徳: "コラーゲンの分子間架橋の解析" 臨床皮膚科. 50. 91-97 (1996)
[Publications] 久保木芳徳: "歯のマトリックスと疾患" 医学のあゆみ. 177. 105-110 (1996)
[Publications] 佐々木勝: "Bone Morphogenetic Protein.(BMP)による歯周組織の再生療法に関する研究" 日歯周誌. 38. 428-446 (1996)
[Publications] Onodera,S.: "Purification of a noncollagenous high molecular weight protein from bovine articular cartilage." Biol.Pharm.Bull.19. 11-17 (1996)
[Publications] Liu,An-jun: "Changes of Noncollagenous Extracellular Matrix in Turkey Leg Thedon During Calcification." J.Hard Tissue Biol.5. 201-206 (1996)
[Publications] Mizuno,M.: "The Osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen,Which promoted cell-attachment of osteoblasts." Biochim.Biophys.Acta. 1310. 97-102 (1996)
[Publications] Fujisawa,R.: "Acidic amino acid-rich sequences as binding sites of osteonectin to hydroxyapatite crystals." Biochim.Biophys.Acta. 1292. 53-60 (1996)
[Publications] Mizuno,M.: "Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and show affinity to collagen." Calcif Tissue Int. 59. 163-167 (1996)
[Publications] Kobayashi,D.: "Time-dependent expression of bone sialoprotein fragments in osteogenesis induced by bone morphogenetic protein." J.Biochem.119. 475-481 (1996)
[Publications] Wada,Y.: "Electrophoretic gels of dentin matrix proteins as diffusion media for in vitro mineralization." J.Dent.Res.75. 1381-1387 (1996)
[Publications] Ono,I.: "Promotion of the Osteogenetic Activity of Recombinant Human Bone Morphogenetic Protein by Basic Fibroblast Growth Factor." J.Cranio.Surg.7. 418-425 (1996)
[Publications] Mizuno,M.: "Osteogenesis by Bone Marrow Stromal Cells Maintained on Type I Collagen Matrix Gels In Vivo." Bone. 20. 101-107 (1997)
[Publications] Tsuruga,E.: "Geometry of cell-substratum controls BMP-induced osteogenesis." J.Biochem.121. 317-324 (1997)
[Publications] Fujisawa,R.: "Attachment of osteoblastic cells to hydroxyapatite crystals by a synthetic peptide (Glu_7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein." Matrix Biol.16. 21-28 (1997)
[Publications] Nifuji,A.: "Perturbation BMP signaling in somitogenesis resulted in vertebral and rib malformations in the axial skeletal formation" J.Bone Minr.Res.12. 332-342 (1997)
[Publications] Masuda,H.: "Bone mass loss due to estrogen deficiency is compensated in transgenic mice overexpressing human osteoblast stimulating factor-1." Biochem.Biophys.Res.Commun. 238. 528-533 (1997)
[Publications] Takita,H.: "Enhancement by bFGF of osteogenesis induced by rhBMP-2 in rats." Eur.J.Oral Sci.105. 588-592 (1997)
[Publications] Liu,A.: "Mechanism of incorporation of histones into bone matrix." J.Hard Tissue Biol. 6. 82-88 (1997)
[Publications] Ohgushi,H.: "Bone induction on the surface of hydroxyapatite ceramics." Bioceramics. 6. 82-88 (1997)
[Publications] 久保木芳徳: "人工歯根膜への道(2)-歯周組織の生化学的再建." バイオインダストリー. 14. 31-44 (1997)
[Publications] 久保木芳徳: "オランダはこうして歯科医療改革に成功した-「歯直し」から「世直し」へ." 北海道歯会誌. 53. 21-31 (1998)
[Publications] Kuboki,Y.: "BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: topology of Osteogenesis." J.Biomed.Mater.Res.39. 190-199 (1998)
[Publications] Kuboki,Y.: "Two new in vitro calcification systems showing the higher calcification of enamel proteins than dentin and bone matrices." Connect.Tissue Res.(in press).
[Publications] Kuboki,Y.: "Regeneration of periodontal ligament and cementum by BMP-applied tissue engineering." Eur.J.Oral Sci.(in press).
[Publications] Fujisawa,R.: "Conformation of dentin phosphophoryn adsorbed on hydroxyapatite crystals." Eur.J.Oral Sci.(in press).
[Publications] Sasano,Y.: "Type X collagen is not localized in hypertrophic or calcified cartilage in the developing rat trachea." Anat.Embryol.(in press).
[Publications] 久保木芳徳: "東海生物の石灰化と硬組織" 東海大学出版会, 312 (1996)

1997 Fiscal Year Annual Research Report

セメント質と歯根膜をともなったハイブリッド型人工歯根の開発

Principal Investigator

久保木 芳徳 北海道大学, 歯学部, 教授 (00014001)

Research Products

[Publications] 久保木芳徳: "コラーゲンの分子間架橋の解析" 臨床皮膚科. 50. 91-97 (1996)

[Publications] 久保木芳徳: "歯のマトリックスと疾患" 医学のあゆみ. 177. 105-110 (1996)

[Publications] 佐々木 勝: "Bone Morphogenetic Protein.(BMP)による歯周組織の再生療法に関する研究" 日歯周誌. 38. 428-446 (1996)

[Publications] Onodera,S.: "Purification of a noncollagenous high molecular weight protein from bovine articular cartilage." Biol.Pharm.Bull.19. 11-17 (1996)

[Publications] Liu,An-jun: "Changes of Noncollagenous Extracellular Matrix in Turkey Leg Thedon During Calcification." J.Hard Tissue Biol.5. 201-206 (1996)

[Publications] Mizuno,M.: "The Osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen,Which promoted cell-attachment of osteoblasts." Biochim.Biophys.Acta. 1310. 97-102 (1996)

[Publications] Fujisawa,R.: "Acidic amino acid-rich sequences as binding sites of osteonectin to hydroxyapatite crystals." Biochim.Biophys.Acta. 1292. 53-60 (1996)

[Publications] Mizuno,M.: "Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and show affinity to collagen." Calcif Tissue Int. 59. 163-167 (1996)

[Publications] Kobayashi,D.: "Time-dependent expression of bone sialoprotein fragments in osteogenesis induced by bone morphogenetic protein." J.Biochem.119. 475-481 (1996)

[Publications] Wada,Y.: "Electrophoretic gels of dentin matrix proteins as diffusion media for in vitro mineralization." J.Dent.Res.75. 1381-1387 (1996)

[Publications] Ono,I.: "Promotion of the Osteogenetic Activity of Recombinant Human Bone Morphogenetic Protein by Basic Fibroblast Growth Factor." J.Cranio.Surg.7. 418-425 (1996)

[Publications] Mizuno,M.: "Osteogenesis by Bone Marrow Stromal Cells Maintained on Type I Collagen Matrix Gels In Vivo." Bone. 20. 101-107 (1997)

[Publications] Tsuruga,E.: "Geometry of cell-substratum controls BMP-induced osteogenesis." J.Biochem.121. 317-324 (1997)

[Publications] Fujisawa,R.: "Attachment of osteoblastic cells to hydroxyapatite crystals by a synthetic peptide (Glu_7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein." Matrix Biol.16. 21-28 (1997)

[Publications] Nifuji,A.: "Perturbation BMP signaling in somitogenesis resulted in vertebral and rib malformations in the axial skeletal formation" J.Bone Minr.Res.12. 332-342 (1997)

[Publications] Masuda,H.: "Bone mass loss due to estrogen deficiency is compensated in transgenic mice overexpressing human osteoblast stimulating factor-1." Biochem.Biophys.Res.Commun. 238. 528-533 (1997)

[Publications] Takita,H.: "Enhancement by bFGF of osteogenesis induced by rhBMP-2 in rats." Eur.J.Oral Sci.105. 588-592 (1997)

[Publications] Liu,A.: "Mechanism of incorporation of histones into bone matrix." J.Hard Tissue Biol. 6. 82-88 (1997)

[Publications] Ohgushi,H.: "Bone induction on the surface of hydroxyapatite ceramics." Bioceramics. 6. 82-88 (1997)

[Publications] 久保木芳徳: "人工歯根膜への道(2)-歯周組織の生化学的再建." バイオインダストリー. 14. 31-44 (1997)

[Publications] 久保木芳徳: "オランダはこうして歯科医療改革に成功した-「歯直し」から「世直し」へ." 北海道歯会誌. 53. 21-31 (1998)

[Publications] Kuboki,Y.: "BMP-induced osteogenesis on the surface of hydroxyapatite with geometrically feasible and nonfeasible structures: topology of Osteogenesis." J.Biomed.Mater.Res.39. 190-199 (1998)

[Publications] Kuboki,Y.: "Two new in vitro calcification systems showing the higher calcification of enamel proteins than dentin and bone matrices." Connect.Tissue Res.(in press).

[Publications] Kuboki,Y.: "Regeneration of periodontal ligament and cementum by BMP-applied tissue engineering." Eur.J.Oral Sci.(in press).

[Publications] Fujisawa,R.: "Conformation of dentin phosphophoryn adsorbed on hydroxyapatite crystals." Eur.J.Oral Sci.(in press).

[Publications] Sasano,Y.: "Type X collagen is not localized in hypertrophic or calcified cartilage in the developing rat trachea." Anat.Embryol.(in press).

[Publications] 久保木芳徳: "東海生物の石灰化と硬組織" 東海大学出版会, 312 (1996)

久保木芳徳北海道大学, 歯学部, 教授 (00014001)

[Publications] 佐々木勝: "Bone Morphogenetic Protein.(BMP)による歯周組織の再生療法に関する研究" 日歯周誌. 38. 428-446 (1996)