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1997 Fiscal Year Final Research Report Summary

Molecular cloning related to skeletal or muscle atrophy under microgravity

Research Project

Project/Area Number 08557151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionKagoshima University

Principal Investigator

KITAJIMA Isao  Kagoshima University Faculty of Medicine, Associate Professor, 医学部, 助教授 (50214797)

Co-Investigator(Kenkyū-buntansha) HANYU Naojiro  Tokuyama, Co.Ltd.Chief.Reseacher, 基礎研究センター, 主任研究員
OHIRA Yoshinobu  Natl.Inst.Fitness and Sports, Kanoya, Professor, 体育学部, 教授 (50185378)
SEMBA Ichiro  Kagoshima University Faculty of Dent, Associate Professor, 歯学部, 助教授 (60145505)
MARUYAMA Ikuro  Kagoshima University Faculty of Medicine Professor, 医学部, 教授 (20082282)
Project Period (FY) 1996 – 1997
KeywordsSpace-flight / rat / bone atrophy / remodeling / microgravity / metal composition / mRNA / cloning
Research Abstract

The effect of 14 days of flight on the vertebrae of rapidly growing rats was studied. No significant difference (p>0.05) was observed between flight rats and ground controls with regard to mechanical hardness or bone mineral density. however, histological examination revealed irregular thickening of the endosteal surface of cortical bones in flight rats, whereas it was uniform in the ground controls. The relative area of lamellar bone showed a significant reduction (p<0.001) in the flight rats. These findings suggest that structural disturbances were due to retardation of endosteal modeling and remodeling. Next, the effects of 14 days of space-flight on beta-adrenoceptor (beta-AR), mitochondrial enzyme activities, and fiber type composition were studied in plantaris muscles. The maximum binding capacity of beta-AR (B max) was significantly lower (>29%) after flight, and the recovery was not complete after 9 days in a 1-G environment. The activity of succinate dehydrogenase (SDH) was measured in muscle samples <5 hr after flight and were found to normalize during 9 days of recovery. It is suggested that the space-flight-induced decrease of B max of beta-AR in plantaris was accompanied by a decreased activity of a mitochondrial inner-membrane enzyme, SDH.Finally, the expression of mRNA in paraspinal muscles between space-flight rats and ground controls was compared using mRNA differential display method in order to identify molecules related to muscle atrophy under microgravity. We found that 70 kDa heat-shock protein (HSP70) and its retated gene, TCP-1, were makedly elevated during 14 days of space-flight, while genes involved in mitochondrial metabolism (mitochondrial adenosine nuclease translocator, mitochondrial cytochrome oxidase subunits), aldolase A and myocyte-specific enhancer binding factor (MEF) were significantly decreased. Some new genes related to muscle atrophy were also detected and we studied the biological functions of these molecules.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Isao Kitajima, Naohiro Hanyu et al.: "Ribozymc-based gene cleavage approach to chronic arthritis associated with human T-cell leukemia virus typeI (HTLV-I) :" Arthritis & Rheumatism. 40. 2118-2127 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isao Kitajima, Naohiro Hanyu, et al: "Efficient transfer of synthetic ribozymes into cells using hemagglutinating virus of Japan (HVJ) -cationic liposomes." J.Biol.Chem.272. 27099-27106 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gen Yamada, Isao Kitajima, et al: "Metal composition and histological analysis of the space flight rat bones" Life Science. 60. 635-642 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jun-Ichiro Nishi Isao Kitajima, et al.: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B :" J.Immunol.158. 247-254 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kitajima, I., Senba, I.et al: "growth disfurbance in vertebral bones of rapidly growing rato following 14 days of spaceflight" Journal of Applied physiology. 81(1). 156-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohira, Y., Yasui, W, et al: "Spaceflight effects on β-adrenoceptor and metabolic properties in rat plantarls" Journal of Applied Physiology. 81(1). 152-155 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikuro Maruyama, Isao Kitajima, et al: "Thrombin activates NF-kB through thrombin receptor" Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIER,Amsterdam,260 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Kitajima, N.Hanyu, K.Kawahara, Y.Soejima, T.Kubo, R.Yamada, Y.Kaneda, I.Maruyama: "Ribozyme-based gene cleavage approach to chronic arthritis associated with human T-cell leukemia virus type I (HTLV-I) : Induction of apoptosis in synoviocytes by ablation of HTLV-I Tax protein." Arthritis.Rheum.40. 2118-2127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Kitajima, N.Hanyu, Y.Soejima, R.Hirano, S.Arahira, S.Yamaoka, R.Yamada, I.Maruyama, Y.Kaneda: "Efficient transfer of synthetic ribozymes into cells using hemagglutinating virus of Japan (HVJ)-cationicl iposomes. Application for ribozymes that target Human T-cell leukemia virus type I tax/rex mRNA." J.Biol.Chem.272. 27099-27106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Yamada, K.Sugimura, S.Nakamura, M-O.Yamada, Y.Okazaki, I.Maruyama, I.Kitajima, T.Minami: "Metal comp[osition and histological analysis of the space flight rat bones." Life Science. 60. 635-642 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Nishi, S.Kanekura, S.Takei, I.Kitajima, T.Nakajima, M.R.Wahid, K.Matsuda, M.Yoshinaga, I.Maruyama, K.Miyata: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B : The Diminant epitope region recognized by intravenous IgG." J.Immounol.158. 247-254 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Kitajima, I.Senba, T.Noikura, K.Kawano, Y.Iwashita, I.Takasaki, I.Maruyama: "Growth disturbance in vertebral bones of rapidly growing 14 days of spaceflight." J.Appl.Physiol.81. 156-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ohira, W.Yasui, F.Kariya, T.Tanaka, I.Kitajima, I.Muruyama, S.Nagaoka.C.Sekiguchi, W.E.Hinds: "Spaceflight effects on beta-adrenoreceptor and metabolic poperties in rat plantaris." J.Appl.Physiol.81. 152-155 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikuro Maruyama, Isao Kitajima, et al: Thrombin activalcs NF-kB through thrombin receptor. Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIRE,Amsterdam, 161-167 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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