1998 Fiscal Year Final Research Report Summary
Development of high sensitive adeno virus detection system
| Project/Area Number |
08557152
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
AZUMA Takachuka Research Institute for Biological Sciences, Professor, 生命科学研究所, 教授 (00028234)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Hideo Toagousei Co.Tukuba Institute, Director, つくば研究所, 所長
ITO Norihiko Yokohama City University Medical School, Research Associate, 医学部, 助手 (80264654)
OHNO Shigeaki Yokohama City University Medical School, Professor, 医学部, 教授 (50002382)
UCHIO Eichi Yokohama City University Medical School, Assistant Professor, 医学部, 講師 (70232840)
MATSUO Katsuhiko Toagousei Co.Tukuba Institute, Investigater, つくば研究所, 主査研究員
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| Project Period (FY) |
1996 – 1998
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| Keywords | monoclonal antibody / Adenovirus / immunoassay / high sensitivity |
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| Research Abstract |
We have prepared hybridomas producing anti-adenovirus monoclonal antibodies (mAbs) after immunization of BALB/c mice wilh adeno virus infected Hela cells. Most of mAbs reacted with all adenovirus types except one. The latter reacted with type 37 and possessed virus neutrolizing activity. Using a pair of mAbs, C14 and C27, we developed sandwitch ELISA. C27 was conjugated with alkalinephosphatase. This system was able to detect any type pf adenovirus with sensitivity 100-1000 fold higher than the others.
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