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1998 Fiscal Year Final Research Report Summary

Development of systems for cell culture and expression vector to generate transgenic marine invertegra

Research Project

Project/Area Number 08558080
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Developmental biology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIMADA Hiraku  Fuculty of Science, Hiroshima University, Professor, 理学部, 教授 (70011559)

Co-Investigator(Kenkyū-buntansha) MACHII Akira  Yamakatsu Pearl Co., Investigator, 養殖研究室, 顧問研究員
淡路 雅彦  水産庁中央水産研究所, 生物機能部・分子生物研究室, 室長
WADA Katsuhiko  National Research Institute of agriculture, Head Investigator, 遺伝育種部, 部長
NAKATSUBO Keiko  Fuculty of Science, Hiroshima University, Assistant, 理学部, 助手 (40192760)
AKASAKA Koji  Fuculty of Science, Hiroshima University, Associate Professor, 理学部, 助教授 (60150968)
MASAHIKO Awaji  National Research Institute of Fisheries Science, Investigator
Project Period (FY) 1996 – 1998
Keywordssea urchin / pearl oyster / transgenic animal / insulator / expression vector / cell culture
Research Abstract

1. Identification of cis-active elements that regulate expression of sea urchin gene :
Several sequence elements that regulate transcription of the sea urchin Ars gene were detected by reporter assay around its transcription start site. Among them between Sox-binding sitebetween -168bp and -162bp is most prominent element. Around -186bp cis-element that represses the promoter activity was detected.
2. Validity of insulator element in gene transfer experiments :
An insulator element present at the upstream of the sea urchin Ars gene showed insulator activity also in cells of Drosophila, mouse and humane
3. Cell culture system for mantle epitermal cells of pearl oyster :
Primary cell culture system for mantle epitermal cells of pearl oyster was established, though they do not proliferate in vitro.
4. Culturing of sea urchin embryonic cells :
Though we succeeded in establishing in vitro culture of cells from sea urchin pluteus larvae, we failed to culture them beyond three days because of difficulty in preventing proliferation of contaminating fungi.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ishii et al: "Hbox1 and Hbox7 are involved in the Pattern Formation of Sea urchin Embryo" Development Growth & Differentiation. 41印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurokawa,et al: "HpEts,an ets-related transcription factor implicated in primary mesenchyme cell differentiation of the sea urchin embryo" Mechanism of Development. 80. 41-52 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawasaki,et al: "Lim1-related homeobox gene(HpLim1) expressed in sea urchin embryo" Development Growth&Differentiation. 41印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyama et al: "Structure and function of a sea urchin orthodenticle-related HpOtx gene" Developmental Biology. 193. 139-145 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsunaga-Nakatsubo et al: "Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNA during early development" International Journal of Developmental Biology. 42. 645-651 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsunaga-Nakatsubo et al: "Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos" Journal of Experimental Zoology. 280. 220-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ishii, et al.: "Hbox1 and Hbox7 are involved in the Pattern Formation of Sea urchin Embryo" Dev.Growth & Differ.41(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.Kurokawa, et al.: "HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation of the sea urchin embryo." Mech.Dev.80. 41-52 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kawasaki, et al.: "Liml-related homeobox gene (HpLim 1) expressed in sea urchin embryo" Dev.Growth & Differ.41(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kiyama, et al.: "Structure and function of a sea urchin orthodenticle-relatedHpOtxgene." Dev.Biol.193. 139-145 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mitsunaga-Nakatsubo, et al.: "Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNA during early development." Int.J.Dev.Biol.42. 645-651 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Mitsunaga-Nakatsubo, et al.: "Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos." J.Exp.Zool.280. 220-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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