1998 Fiscal Year Final Research Report Summary
Development of systems for cell culture and expression vector to generate transgenic marine invertegra
| Project/Area Number |
08558080
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Developmental biology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SHIMADA Hiraku Fuculty of Science, Hiroshima University, Professor, 理学部, 教授 (70011559)
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| Co-Investigator(Kenkyū-buntansha) |
MACHII Akira Yamakatsu Pearl Co., Investigator, 養殖研究室, 顧問研究員
淡路 雅彦 水産庁中央水産研究所, 生物機能部・分子生物研究室, 室長
WADA Katsuhiko National Research Institute of agriculture, Head Investigator, 遺伝育種部, 部長
NAKATSUBO Keiko Fuculty of Science, Hiroshima University, Assistant, 理学部, 助手 (40192760)
AKASAKA Koji Fuculty of Science, Hiroshima University, Associate Professor, 理学部, 助教授 (60150968)
MASAHIKO Awaji National Research Institute of Fisheries Science, Investigator
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| Project Period (FY) |
1996 – 1998
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| Keywords | sea urchin / pearl oyster / transgenic animal / insulator / expression vector / cell culture |
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| Research Abstract |
1. Identification of cis-active elements that regulate expression of sea urchin gene :
Several sequence elements that regulate transcription of the sea urchin Ars gene were detected by reporter assay around its transcription start site. Among them between Sox-binding sitebetween -168bp and -162bp is most prominent element. Around -186bp cis-element that represses the promoter activity was detected.
2. Validity of insulator element in gene transfer experiments :
An insulator element present at the upstream of the sea urchin Ars gene showed insulator activity also in cells of Drosophila, mouse and humane
3. Cell culture system for mantle epitermal cells of pearl oyster :
Primary cell culture system for mantle epitermal cells of pearl oyster was established, though they do not proliferate in vitro.
4. Culturing of sea urchin embryonic cells :
Though we succeeded in establishing in vitro culture of cells from sea urchin pluteus larvae, we failed to culture them beyond three days because of difficulty in preventing proliferation of contaminating fungi.
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