1997 Fiscal Year Final Research Report Summary
Study on expression system of C4 photosynthetic genes.
| Project/Area Number |
08640824
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUOKA Makoto Nagoya BioScienceSenter Professor, 生物分子応答研究センター, 教授 (00270992)
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| Project Period (FY) |
1996 – 1997
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| Keywords | C4 photosynthesis / Pyruvate orthosphate dikinase / Phosphoenolpyruvate carboxylase / Gene evolution / Gene expression |
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| Research Abstract |
C3 plants including many agronomically important crops exhibit a lower photosynthetic efficiency due to inhibition of photosynthesis by O_2 and the associated photorespiration. C4 plants had evolved the C4 pathway to overcome low CO_2 and photorespiration. This review first focuses on the generation of a system for high level expression of the C4-specific gene for pyruvate, orthophosphate dikinase (Pdk), one of the key enzyme in C4 photosynthesis. Based on the results with transgenic rice plants, we have demonstrated that the regulatory system controlling the Pdk expression in maize is not unique to C4 plants but rice (C3 plant) posses a similar system. Second, we discuss the possibility of the high level expression of maize C4-specific genes in transgenic rice plants Introduction of the maize intact phosphoenolpyruvate carboxylase gene (Ppc) caused 30-100 fold higher PEPC activities than non-transgenic rice. These results demonstrated that intact C4-type genes are available for high level expression of C4 enzymes in rice plants.
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[Publications] Makino, A., Shimada, T., Takumi, S., Kaneko, K., Matsuoka, M., Shimamoto, K., Nakano, H., Miyao-Tokutomi, M., Mae, T.and Yamamoto, N.: "Does decrease in ribulose-1,5-bisphosphate carboxylase by antisenserbcs leads to a higher N-use efficiency of hotosynthesis under conditionsof stuating CO2 and light in rice plants?" Plant Physiol.114. 483-491 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Imaizumi, N., Ku, M.S.B., Ishihara, K., Samejima, M., Kaneko, S.and Matsuoka M.: "Characterization of the gene for pyruvate, orthophosphate dikinase from the C3 plant rice and comparison of structure and expression between C3 and C4 ppdk genes." Plant Mol.Biol.34. 701-716 (1997)
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「研究成果報告書概要(欧文)」より
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