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1997 Fiscal Year Final Research Report Summary

Nuclear encoded chloroplast ribosomal protein genes ; genomic structure and organ-specific gene expression.

Research Project

Project/Area Number 08660004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

TSUTSUMI Nobuhiro  Univ.Tokyo, Grad.Schl.Agr.Life Sci., Assoc. Prof., 大学院・農学生命科学研究科, 助教授 (00202185)

Project Period (FY) 1996 – 1997
Keywordschloroplast ribosomal protein / RFLP mapping / gene expressionon / rice
Research Abstract

A cDNA library was constructed with poly(A)^+RNA prepared from 10-day-old rice seedlings. I have isolated three nuclear genes, rpl9, rpl12-1, rpl12-2, rpl13, rpl15, rp121, rpl24, rpl28, rps1 and rps9, that encode chloroplast ribosomal proteins L9, L12, L13, L15, L21, L24, L28, S1 and S9 respectively. Using these clones as probes, these nuclear encoded genes were located on molecular linkage map of rice. Seven loci were located on 5 differnt chromosomes of rice. They were not clustered but dispersed throughout the rice genome.
Expression analysis of rpl12-1, rpl12-2, rpl13, rpl24 by Northern blotting reveals that these genes were expessed in leaves of 10-day-old rice seedlings regardless of the presence of light, but not in roots. These data suggest that nuclear encoded ribosomal protein genes were expressed in an organ specific manner.
The genomic clones of rpl13 and rpl24 were isolated, and transcription start sites were determined by S1-nulcease mapping and primer extension. It is suggested that both genes have multiple sites of transcription initiation, and that each site was employed for transcription initiation differently in specific organ.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 堤 伸浩: "Molecular cloning and nucleotide sequencing of nuclear genes coding for the chloroplast ribosomal protein L13,L24,L28 of rice(Oryza sativa L.)." Plant Science. 15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊藤 裕介: "Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日下 宗規: "Characterization of two rice genes for nuclear-encoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor.Appl.Genet.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金澤 章: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes Genet.Syst.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有村 慎一: "Genomic structures and the 5´-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice(Oryza sativa L.)." Breed.Sci.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsutsumi N.: "Molecular cloning and nucleotide seqrencing of nuclear genes coding for the chloroplast ribosomal protein L13, L24, L28 of rice (Oryza sativa L.)" Plant Science. 15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itoh Y.: "Transcript levels of tendem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Itoh Y.: "Transcript levels of tendem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)Kusaka M.:

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] "Characterization of two rice genes for nuclearencoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor. Appl. Genet. ( in press ).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanezawa A.: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes & Genet. Syst.( in press ).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arimura S.: "Genomic structures and the 5'-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice ( Oryza sativa L.)" Breed. Sci.( in press ).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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