1997 Fiscal Year Final Research Report Summary

RT-PCR Expression analysis of ACC oxidase gene and ACC synthase gene in melon leaf in field condition.

Research Project

Project/Area Number	08660041
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	園芸・造園学
Research Institution	OSAKA PREFECTURE UNIVERSITY
Principal Investigator	FURUKAWA Hajime OSAKA PREFECTURE UNIVERSITY,College of Agriculture, Assistant professor, 農学部, 講師 (40240957)
Co-Investigator(Kenkyū-buntansha)	IMABORI Yoshihiro OSAKA PREFECTURE UNIVERSITY,College of Agriculture Research Associate Associate, 農学部, 助手 (40254437)
Project Period (FY)	1996 – 1997
Keywords	Ethylene / Melon / ACC oxidase gene / ACC synthase gene / Gene expression / RT-PCR
Research Abstract	We investigated the expression pattem of the ME-ACS 1 and CM-ACO 1 genes in the response to various inducers of gene expression in field condition. (1) The melon actin gene is useful internal standard, compare with the melon GAPDH gene.. (2) Results of RT-PCR showed that the expression of both genes were induced by wounding, IAA,Ethylene in field condition. (3) Both genes were induced by heat shock stress (38ﾟC) with in 10 min, expression reaching a maximum at 1 hr.and decreasing until 6 h. (4) After heat shock for 1h.melon plats were returned to 25ﾟC,ME-ACS 1 mRNA level gradually decreased, but level of CM-ACO 1 mRNA rewired high for 6 h. (5) ME-ACS 1 mRNA level was constant during leaf senescence, but CM-ACO 1 mRNA level was gradually decreased.