1997 Fiscal Year Final Research Report Summary
Studies on liver detoxifying enzyme inducers in food
| Project/Area Number |
08660153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食品科学・栄養科学
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| Research Institution | Nagoya University |
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Principal Investigator |
UCHIDA Koji Nagoya University, Agricultural Science, Associate Professor, 農学部, 助教授 (40203533)
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| Co-Investigator(Kenkyū-buntansha) |
OSAWA Toshihiko Nagoya University, Agricultural Science, Professor, 農学部, 教授 (00115536)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Food components / Liver detoxiying enzymes / Glutathione S-transferase / Rat hepatocytes / Phenolic antioxidants / t-Butylhydroquinone / BHA / Oxiative stress |
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| Research Abstract |
Regulation of the GST-Yp transcript has been studied extensively. Recent transgenic rat experiments have revealed that a trans mechanism is involved in the activation of this gene during chemical hepatocarcinogenesis. We found that the promoter of GST gene contains TATA box, TRE (from-61 to -54), sirencer (from -396 to -140). Tretatment of RL34 cells to tBHQ resulted in the changes in the DNA binding activity of several redox-active transcription factors including AP-1. Sp1, and NF-kB.Western blot analysis of these transcription factors revealed that tBHQ induced de novo sysnthesis of these proteins. Taken together, tBHQ and other food-derived inducers may act on the promoter of genes of detoxifying enzymes by way of redox regulationmechanism.
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