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1997 Fiscal Year Final Research Report Summary

Roles of the plasma membrane in Ca^<2+> oscillations in smooth muscle cells

Research Project

Project/Area Number 08660361
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionGifu University

Principal Investigator

KOMORI Seiichi  Gifu Univ., Faculty of Agriculture, Professor, 農学部, 教授 (70195866)

Co-Investigator(Kenkyū-buntansha) UNNO Toshihiro  Gifu Univ., Faculty of Agriculture, Research Associate, 農学部, 助手 (90252121)
OHASHI Hidenori  Gifu Univ., Faculty of Agriculture, Professor, 農学部, 教授 (40001531)
Project Period (FY) 1996 – 1997
KeywordsSmooth muscle / Muscarinic receptor / Calcium release / Calcium store / Calcium oscillations / Inositol trisphosphate / G protein
Research Abstract

The present study was made to elucidate the role of the plasma membrane and intracelullar Ca^<2+> stores in oscillations in cytosolic Ca^<2+> concentration following stimulation of muscarinic receptors in single smooth muscle cells of guinea-pig ileum.
1. The muscarinic receptor-mediated Ca^<2+> oscillations occur differentially because of Ca^<2+> influx across the plasma membrane associated with action potential discharge and of Ca^<2+> release from internal stores by a second messenger, inositol trisphosphate.
2. Ca^<2+> store-derived Ca^<2+> oscillations require a sustained Ca^<2+> influx for their persistence and regulation of the oscillation frequency. The L-type of Ca^<2+> channel and an as yet unidentified Ca^<2+> channel may be involved in the sustained Ca^<2+> influx.
3. The plasma membrane-derived type of Ca^<2+> oscillation may have no or little contribution from Ca^<2+>-induced Ca^<2+> release ; Ca^<2+> entering the cell during action potential discharge activtes ryanodine-sensitive, Ca^<2+>-gated Ca^<2+> channels in the internal store to release Ca^<2+> from there.
4. During generation of Ca^<2+> store-derived Ca^<2+> oscillations, membrane potential oscillates coincidentally in time with them. The membrane potential oscillations result each from potentiation of the activity of muscarinic receptor-operated nonselective cation channels by a rise in cytosolic Ca^<2+> concentration.
The results of no.1,2 and 3 have been published in international technical journals and those of no.4 are under submission.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] S.Komori: "Modulation of carbachol-induced [Ca^<2+>] i oscillations by Ca^<2+> influx in single intestinal smooth muscle cells." British journal of Pharmacology. 119. 245-252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kohda: "Carbachol-induced [Ca^<2+>] i oscillations in single smooth muscle cells of guinea-pig ilem." Journal of Physiology. 492. 315-328 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kohda: "Characterization of action potential-triggered [Ca^<2+>]i transient in single smooth muscle cells of guinea-pig ileum." British journal of Pharmacology. 122. 477-486 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Komori: ""Smooth Muscle Excitation" ed. by T.B.Bolton & T.Tomita" Academic Press Ltd.,London MW17DX,UK., 9 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Komori et al.: "Modulation of carbachol-induced [Ca^<2+>] i oscillations by Ca^<2+> influx in single intestinal smooth muscle cells" Br.J.Pharmacol.119. 245-252 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kohda et al.: "Carbachol-induced [Ca^<2+>] i oscillations in single smooth muscle cells of guinea-pig ileum" J.Physiol.492. 315-328 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kohda et al.: "Characterization of action potential-triggered [Ca^<2+>] i transients in single smooth muscle cells of guinea-pig ileum" Br.J.Pharmacol.122. 477-486 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Komori et al.: Mechanisms of cytosolic Ca2+ oscillations following muscarinic receptor activcation in single smooth muscle cells.In "Smooth Muscle Excitation" Bolton, T.B.and Tomita, T.eds.Academic Press, London, 205-213 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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