• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1997 Fiscal Year Final Research Report Summary

Analysis of signal transduction pathway during seed germination

Research Project

Project/Area Number 08660411
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionFukui Prefectural University

Principal Investigator

IWASAKI Yukimoto  Dept.of Bioscience, Fukui Prefectural University, Associate Professor, 生物資源学部, 助教授 (20193732)

Co-Investigator(Kenkyū-buntansha) ASAHI Tadashi  Dept.of Bioscience, Fukui Prefectural University, Professor, 生物資源学部, 教授 (10023392)
Project Period (FY) 1996 – 1997
KeywordsG protein / in situ hybridization / plant / rice / seed / signal transduction
Research Abstract

1.The expression pattern of the putative alpha subunit of a heterotrimeric G protein in rice seeds was investigated during germination and flowering stages. The amount of the transcript for the alpha subunit was observed to increase rapidly from 2nd to 5th day after germination, followed by a gradual increase during growth. The cells expressing the mRNA for RGA1 were analyzed by in situ hybridization with digoxigenin-labeled RNA probes prepared with parts of RGA1 cDNA as the templates. The mRNA was detected in the root tip, scutellum, phloem but not in the root cap and xylem during germination. It was also detected in rachilla, nucellus, and paricape during rapening stage. These results suggest that RGA1 may take part in cell division and/or material transport.
2.A recombinant protein with the cDNA that encodes the putative alpha subunit of G protein was synthesized in Escherichia coli. The purified recombinant protein (rGricealpha) showed an apparent molecular mass of 45 kDa and bound guanosine 5'-(3-O-thio) triphosphate with an apparent association constant (k_<app>) of 0.36. The rGricealpha was ADP-ribosylated by activated cholera toxin. Monoclonal antibodies raised against rGricealpha reacted with a 45-kDa polypeptide localized in the plasma membrane of rice seedlings. A 45-kDa polypeptide in the plasma membrane, as well as rGricealpha, was ADP-ribosylated by activated cholera toxin. The properties observed are similar to those reported for the alpha subunits of heterotrimeric G proteins in animals, suggesting that the putative alpha subunit is truly the alpha subunit itself.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Iwasaki, Y., Kato, T., Kaido, T., Ishikawa, A.and Asahi, T.: "Characterization of the putative α subunit of a heterotrimeric G protein in rice." Plant Mol.Biol.34. 563-572 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa, A., Tubouchi, H., Iwasaki, Y.and Asahi, T.: "Molecular cloning and characterization of cDNA for an α subunit of G protein from rice" Plant Cell Physiol.36(2). 353-359 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaki Y., Ishikawa A., Kato T., Nanbu N., and Asahi T.: "Structure and function of putative heterotrimeric G proteins in higher plants" in "Recent Research Developments in Agricultural and Biological Chemistry 1" Edited by S.G.Pandalai. Publisher Research Signpost, 12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩崎 行玄・海藤 敏雄: "融合蛋白質を用いた特異抗体の調製" 植物細胞工学シリーズ9号、植物の蛋白質実験プロトコール,

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaki, Y., Kato, T., Kaido, T., Ishikawa, A.and Asahi, T.: "Characterization of the putative alpha subunit of a heterotrimeric G protein in rice." Plant Mol.Biol.34. 563-572 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaki Y., Ishikawa A., Kato T., Nanbu N., and Asahi T.: "Structure and function of putative heterotrimeric G proteins in higher plants in "Recent Research Developments in Agricultural and Biological Chemistry 1"" Edited by S.G.Pandalai.Publisher Research Signpost. 59-70 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, A., Tubouchi, H., Iwasaki, Y.and Asahi, T.: "Molecular Cloning and characterization of cDNA for an alpha subunit of G protein from rice." Plant Cell Physiol.36(2). 353-359 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi