ULTRASTRUCTURAL ANALYSIS OF HUMAN IgE RECEPTOR BY ATOMIC FORCE MICROSCOPY

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 08670005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: CHIBA UNIVERSITY
• Principal Investigator: TATSUOKA Hozumi DEPARTMENT OF ANATOMY,CHIBA UNIVERSITY,SCHOOL OF MEDICINE, 医学部, 助教授 (10125940)
• Co-Investigator(Kenkyū-buntansha): RA Chisei DEPARTMENT OF IMMUNOLOGY,JUNTENDO UNIVERSITY,SCHOOL OF MEDICINE, 医学部免疫学, 講師 (60230851)
• Project Period (FY): 1996 – 1997
• Keywords: Immunoglobulin Ereceptor / Atomic force microscopy / Low angle rotary shadowing technique

Research Abstract

Surface structures of the extracellular domain of alpha-chain of human IgE receptor was analyzed by atomic force microscopy (AFM) and low angle rotary shadwing replica technique. We observed the surface structure of the IgE receptor, IgE,and IgE receptor-IgE complex. The receptor were looked like almost spherical-shape by AFM,and doughnut-shape by the rotary shadowing replica. In the receptor-IgE complex IgE itself became more massive at one end of the molecule. These observations may contribute ot understand some aspects of the conformational change by ligand-receptor interaction.