1997 Fiscal Year Annual Research Report

末梢神経再生におけるシュワン細胞と軸索の相互作用-共焦点レーザー顕微鏡による解析

Research Project

Project/Area Number	08670020
Research Institution	KYUSHU UNIVERSITY
Principal Investigator	川渕優九州大学, 医学部, 教授 (80037449)
Co-Investigator(Kenkyū-buntansha)	灘修身九州大学, 医療短大, 教授 (20040727) 中村桂一郎九州大学, 医学部, 助教授 (20172398)
Keywords	神経筋接合部 / 神経再生 / 軸索誘導 / 免疫組織化学 / 加齢 / 細胞外物質 / シュワン細胞 / 接着分子
Research Abstract	本研究課題は坐骨神経圧挫後、下肢筋の運動終板再神経支配においてシュワン細胞-軸索-細胞外物質の構造的相関が若年期(3月齢ラット)と老年期(24月齢ラット)でいかなる差異を示すかを検索することである。シュワン細胞、軸索、細胞外物質(tenascin)、接着分子(N-CAM)の各々を標識するmonoclonalまたはpolyclonal抗体を組み合わせ二重染色を行い、共焦点レーザー顕微鏡により観察した。また、これらの抗体をalpha-bungarotoxinとも組み合わせ、アセチルコリン受容器との構造的相関を解析した。老年期における特徴として前年度(平成8年度)には、次の所見を認めた。1)神経再生の早期から変性シュワン細胞が出現する、2)再生軸索は神経束から運動終板に伸びていくさい、不規則な走行や崩壊を示す、3)シナプス再構築の進展は運動終板の構成要素(終末軸索、終末シュワン細胞、アセチルコリン受容器)間で不均一である。4)N-CAM,tenascinはシュワン細胞の周縁に存在するものと考えられるが、免疫組織化学的には陽性部位の分布や形状における変化が観察される。これらの所見は軸索誘導、シナプスの再構築・維持における異常を示唆する。平成9年度にはこのような結果にもとづき論文を作成し、現在投稿中である。シナプス再構築・維持における成熟度の不均一性を更に解析するため、神経終末、終末シュワン細胞、アセチルコリン受容器の各部位のサイズおよび重複部位の面積を経時的に計測している。また、基底膜物質(laminin,proteoglycan)、髄鞘形成との関連についてもデータが必要であり、免疫組織化学的に検索している。

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] Masaru Kawabuchi: "The effect of aging on the morphological nerve changes during nerve regeneration" Restorative Neruorolgy & Neruoscience. (accepted).
[Publications] A.T.M.S.Islam: "Expression of NOS,P PSA-N-CAM and S100 protein in the granule cell maturation pathway of the adult guinea pig forebrain" Developmental Braine Research. (in press).
[Publications] A.T.M.S.Islam: "Olfactory granule cell dispersion in the adult guinea pig forebrain" Brain Research Protocols. (in press).
[Publications] Hiroki Tohma: "Three dimensional structure of c-Kit-positive cellular networks in the guinea pig small intestine and colon" Cell and Tissue Research. (in press).
[Publications] Kazuho Hirata: "Postnatal development of Schwann cells at neruomuscular junctions,with special rererence to synapse elimination" Journal of Neruocytology. 26. 799-809 (1997)
[Publications] A.T.M.S.Islam: "Expression of N-CAM-H ans S100 protein in the rostral migratory stream of the adult guinea pig" Acta Histochemica et Cytochemica. 30. 315-318 (1997)
[Publications] Masaru Kawabuchi: "Neuronal PSA-N-CAM immunopositive clusters in the adult guinea pig forebrain" Abstracts Society for Neuroscience 27th Annulal Meeting. 23. 871 (1997)
[Publications] Hiroyuki Isayama: "Distribution and co-localization of nitric oxide synthase and arginosuccinate synhthetase in the cat hypothalamus" Archieves of Histology and Cytology. 60. 477-492 (1997)
[Publications] Masaru Kawabuchi: "The effect of aging on reinnervation to the motor endplate" Acta Anatomica Japonica. 72. 360 (1997)
[Publications] Masaru Kawabuchi: "A morphological study of thymus stromal cells(TEL-2 cell)which play a role in the elimination of doulble positive immature thymocytes by phagocytosis" Anatomical Record. 244. 271-283 (1996)
[Publications] Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation of the adult guinea pig olfactory bulb" Abstracts Society for Neuroscience 26th Annulal Meeting. 22. 980 (1997)
[Publications] Masaru Kawabuchi: "Morphological features of collateral innervation and polyneuronal innervationin the skeletal muscle of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

1997 Fiscal Year Annual Research Report

末梢神経再生におけるシュワン細胞と軸索の相互作用-共焦点レーザー顕微鏡による解析

Principal Investigator

川渕 優 九州大学, 医学部, 教授 (80037449)

Research Products

[Publications] Masaru Kawabuchi: "The effect of aging on the morphological nerve changes during nerve regeneration" Restorative Neruorolgy & Neruoscience. (accepted).

[Publications] A.T.M.S.Islam: "Expression of NOS,P PSA-N-CAM and S100 protein in the granule cell maturation pathway of the adult guinea pig forebrain" Developmental Braine Research. (in press).

[Publications] A.T.M.S.Islam: "Olfactory granule cell dispersion in the adult guinea pig forebrain" Brain Research Protocols. (in press).

[Publications] Hiroki Tohma: "Three dimensional structure of c-Kit-positive cellular networks in the guinea pig small intestine and colon" Cell and Tissue Research. (in press).

[Publications] Kazuho Hirata: "Postnatal development of Schwann cells at neruomuscular junctions,with special rererence to synapse elimination" Journal of Neruocytology. 26. 799-809 (1997)

[Publications] A.T.M.S.Islam: "Expression of N-CAM-H ans S100 protein in the rostral migratory stream of the adult guinea pig" Acta Histochemica et Cytochemica. 30. 315-318 (1997)

[Publications] Masaru Kawabuchi: "Neuronal PSA-N-CAM immunopositive clusters in the adult guinea pig forebrain" Abstracts Society for Neuroscience 27th Annulal Meeting. 23. 871 (1997)

[Publications] Hiroyuki Isayama: "Distribution and co-localization of nitric oxide synthase and arginosuccinate synhthetase in the cat hypothalamus" Archieves of Histology and Cytology. 60. 477-492 (1997)

[Publications] Masaru Kawabuchi: "The effect of aging on reinnervation to the motor endplate" Acta Anatomica Japonica. 72. 360 (1997)

[Publications] Masaru Kawabuchi: "A morphological study of thymus stromal cells(TEL-2 cell)which play a role in the elimination of doulble positive immature thymocytes by phagocytosis" Anatomical Record. 244. 271-283 (1996)

[Publications] Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation of the adult guinea pig olfactory bulb" Abstracts Society for Neuroscience 26th Annulal Meeting. 22. 980 (1997)

[Publications] Masaru Kawabuchi: "Morphological features of collateral innervation and polyneuronal innervationin the skeletal muscle of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

川渕優九州大学, 医学部, 教授 (80037449)