1997 Fiscal Year Final Research Report Summary
The destiny of the neural crest cells where the homeobox gene was ectopically expressed.
| Project/Area Number |
08670036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tokai University |
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Principal Investigator |
TANIGUCHI Yasushi Tokai University School of Medecine Department of Molecular LIfe Science Lecturer, 医学部, 講師 (30207188)
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| Project Period (FY) |
1996 – 1997
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| Keywords | homeobox / HOXD3 / cell adhesion / integrin / cadherin / Wnt-1 / neural crest / transgenic mouse |
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| Research Abstract |
I isolated the human homeobox gene HOXD3 on chromosome 2 from a human genomic library and determined its nucleotide sequence. To study the role of HOXD3 gene in erythropoiesis, expression vectors containing the HOXD3 gene in the sense or antisense orientation were introduced into HEL cells. The sense transfectants overexpressing the HOXD3 gene attached more efficiently to fibronectin and collagen than did the antisense transfectants and parental HEL cells. Northern blot analysis showed that integrin beta3 mRNA levels were significantly increased in the HEL cells overexpressing the HOXD3 gene, whereas the integrin beta1 and alphaIIb mRNA levels did not show a distinct correlation with HOXD3 mRNA levels. Fluorescense-activated cell sorting analysis showed that the sense transfectants overexpressing the HOXD3 gene expressed increased levels of integrin alphaIIbbeta3 (GP IIbIIIa) complex as compared with the parental HEL cells and antisense transfectants. In addition, reverse transcriptase-mediated polymerase chain reaction (RT-PCR) analysis revealed that R-cadherin mRNA Ievels were significantly increased in the antisense transfectants expressing very low levels of the HOXD3 mRNA as compared to the sense transfectants and parental HEL cells. These results implicate the homeobox gene HOXD3 in the regulation of cell adhesion process. To verify the regulation by the HOXD3 also in developing mouse embryos, I made transgenic mice where the human HOXD3 gene was specifically expressed in the neural crest cells and analyzed the differentiation and adhessiveness of the cells.
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[Publications] Taniguchi, Y., Katsumata, Y., Koido, S., Suemizu, H., Yoshimura, S., Moriuchi, T., Kagotani, K., Taguchi, H., Imanishi, T., Gojobori , T., and Inoko, H.: "Cloning, sequencing, and chromosomal localization of two tandemly arranged human pseudogenes for the proliferating cell nuclear antigen (PCNA)" Mamm.Genom. 7. 906-908 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuzaka, Y., Kagotani, K., Okumura, K., Kimura, M., Inoko H., and Taniguchi, Y.: "Isolation and structural analysis of the mouse Nat (Ring3) gene in the MHC class II region." Nucleic Acids Symp.Ser.35. 225-226 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Taniguchi, Y., Matsuzaka, Y., Fujimoto, H.Miyado, K., Kohda A., Okumura, K., Kimura, M., and Inoko, H.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. (in press). (1998)
Description
「研究成果報告書概要(欧文)」より
