1997 Fiscal Year Final Research Report Summary
Mechanism of Bone Growth in Length-Vascular Invasion and Formation of Chondroclast
| Project/Area Number |
08670037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Jikei university School of Medicine |
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Principal Investigator |
FUKUSHIMA Osamu Jikei University School of Medicine Department of Anatomy (1) Lecturer, 医学部, 講師 (60173332)
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| Project Period (FY) |
1996 – 1997
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| Keywords | GROWTH PLATH / ENCHONDRAL OSSIFICATION / CHONDROCLAST / HISTOCHEMISTRY / PROTEASE / ELECTRON MICROSCOPE |
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| Research Abstract |
The present study was planned for investigating mechanisms of bone growth in length. The author employed 3-week old chicken because they showed longer vascular invasion in the growth plate than that in the mammmalia. This is a benefit for observation with electron microscope.
Serial Epon simithin sections showed the looped capillary at the tip of vascular invasion. There was no cartilage resorbing cell. The peptidase and lysosomal enzyme histochemical results also supported it. Multinuclear large cells showing small vesicles in the cytoplasm and no ruffled border were seen in the peri-vascular space at vascular invasion lower than the tip. An alkaline pnitrophenylphosphatase activity was positive on the small vesicle membrane, resulted that they were a kind of chondroclasts. At further lower portion, attached chondroclasts were observed on the surface of the cartilage matrix. There, a mono-nuclear prechondroclast fuesd to an attached chondroclast. The author identified there with "chondroclast formation site".
From these findings, the author concluded that a chondroclasts was yielded at the chondroclast formation site, and moved in the peri-vascular space toward to the tip as a moving chondroclast when the bone grew in length.
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