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1997 Fiscal Year Final Research Report Summary

Identification of troponin subunits which regulate the length dependent change in the Ca sensitivity in cardiac muscles

Research Project

Project/Area Number 08670063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionJikei University School of Medicine

Principal Investigator

KURIHARA Satoshi  Jikei University School of Medicine Department of Physiology (II) Professor, 医学部, 教授 (90057026)

Project Period (FY) 1996 – 1997
Keywordscardiac muscle / muscle length / troponin-C, / Ca sensitivity / Ca ion, / troponin-I, / acidosis, / isoproterenol
Research Abstract

Cardiac muscle contraction is significantly altered by a change in initial muscle length before contraction. This is considered to be due to the crossbridge-dependent change in the affinity of troponin-C (Tn-C) for Ca ion. The hypothesis was examined to measure the relation between Ca ion concentration and tension (pCa-tension relation) at different muscle lengths in intact and skinned preparations of mammalian cardiac muscles. In intact preparations, the papillary muscles treated with ryanodine were repetitively stimulated and tetanus was induced for measuring pCa-tension relation at different muscle lengths in the presence or absence of isoproterenol (ISO). ISO increased the length-dependent shift of pCa-tension relation. Thin trabeculae were treated with Triton X and stored in 50% glycerol containing relaxing solution. pCa-tension relations at different muscle lengths were measured before and after phosphorylation by A-kinase. After phosphorylation, length-dependent shift of pCa-tension relation was lessened. These results suggest that Tn-I and/or C-protein which can be phosphorylated by A-kinase might be involved in the signal transduction from crossbridges to Tn-C.The different effects of A-kinase dependent phosphorylation in intact and skinned preparation should be further investigated.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Kurihara, S.: "Cross-bridge-dependent changes in the intracellular Ca^<2+> concentration in mammalian cardiac muscles" Japanese Heart Journal. 37. 143-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Regulation of cardiac muscle contraction by intracellular Ca^<2+> concentration change" Tokyo Jikei Medical Journal. 111. 265-278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Effects of developed tension on the time course of Ca^<2+> transients in ferret myocardium" Cardiovascular Research. 32. 384-390 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Intracellular Ca^<2+> transients in response to step length changes in aequorin-injected ferret papillary muscle" Pathophysiology. 253-261 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Physiological significance of the change in the Ca^<2+> sensitivity of the contractile elements in cardiac muscle" Molecular and Cellular Mechanisms of Cardio-vascular Regulation. 281-290 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Contractility of cardiac muscle and Ca ion" Clinical Calcium. 6. 9-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Length dependence of Ca^<2+>-tension relationship in aequorin-injected ferret papillary muscles" American Journal of Physiology. 273. H1068-H1074 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Effects of acidosis on Ca^<2+> sensitivity of contractile elements in intact ferret myocardium" American Journal of Physiology. 274 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Intracellular Ca^<2+> movement and inotropic agents in cardiac muscles" Clinical Calcium. 7. 12-17 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: "Calcium sensitivity" Respiration and Circulation. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurihara, S.: Current Methods in Muscle Physiology. (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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