1998 Fiscal Year Annual Research Report

ヒト軟部腫瘍における染色体および遺伝子異常の解析

Research Project

Project/Area Number	08670229
Research Institution	University of Occupational and Environmental Health(UOEH)
Principal Investigator	橋本洋産業医科大学, 医学部, 教授 (10128069)
Co-Investigator(Kenkyū-buntansha)	森光洋介産業医科大学, 医学部, 講師 (90258476) 久岡正典産業医科大学, 医学部, 助教授 (40218706)
Keywords	軟部腫瘍 / 染色体異常 / 遺伝子異常 / 染色体分析 / RT-PCR / 融合遺伝子 / パラフィン包埋組織
Research Abstract	ホルマリン固定パラフィン包埋組織より抽出したRNAを用いたRT-PCR法により、腫瘍組織の多くからmRNAが抽出でき、粘液型・円形細胞型脂肪肉腫、滑膜肉腫、およびEwing/PNET群でそれぞれに特徴的な融合遺伝子産物を高頻度に検出することに成功した平成9年度までの結果をふまえて、これまではその診断を確定することが困難であった肺の滑膜肉腫においてホルマリン固定パラフィン包埋組織よりSYT-SSX融合遺伝子を同定することができ、この方法が臨床的に非定型的な症例の確定病理診断において強力な手技となることを証明した。さらに、隆起性皮膚線維肉腫のホルマリン固定パラフィン包埋組織より特異的融合遺伝子産物COLlAl-PDGFBの検出を試みたところ、73%の症例にCOLlAl-PDGFBが検出でき、それらの塩基配列の解析により、融合遺伝子の切断点は症例により様々であることが判明した。その病理診断学的および腫瘍発生学的意義を検討中である。また、後腹膜にみられる粘液型脂肪肉腫の多くは組織学的に粘液型悪性線維性組織球腫に類似しており、染色体核型分析で高分化型脂肪肉腫にしばしば認められる環状あるいはマーカー染色体を検出したこと、パラフィン包埋組織を用いたRT-PCR法では粘液型脂肪肉腫に特徴的なTLS/FUS-CHOP融合遺伝子を認めなかったことおよび粘液型悪性線維性組織球腫様の部分ではp53遺伝子産物の異常の頻度が高かったことより、これらの症例では染色体・遺伝子変異も古典的粘液型脂肪肉腫と異なり、高分化型ないし脱分化型脂肪肉腫に近い性質を有する腫瘍であると考えられた。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Masanori Hisaoka: "Diagnostic implication of Kaposi's sarcoma-associated herpesvirus with special reference to the distinction between spindle cell hemangioendothelioma and Kaposi's sarcoma." Archives of Pathology & Laboratory Medicine. 122.1. 72-76 (1998)
[Publications] 青木隆敏: "粘液状軟部腫瘍の鑑別診断:パラフィン包埋組織を用いたFISH法の応用" 病理と臨床. 16.2. 161-166 (1988)
[Publications] Masanori Hisaoka: "Intranodal palisaded myofibroblastoma with so-called amianthoid fibers: A report of two cases with a review of the literature." Pathology International. 48.4. 307-312 (1998)
[Publications] Yosuke Morimitsu: "Dermatofibrosarcoma protuberans and its fibrosarcomatous variant with areas of myoid differentiation:A report of three cases." Histopathology. 32.6. 547-551 (1998)
[Publications] Masanori Hisaoka: "Dermatofibrosarcoma protuberans with fibrosarcomatous areas: Molecular abnormalities of the p53 pathway in fibrosarcomatous transformation of dermatofibrosarcoma protuberans" Virchows Archiv. 433.4. 323-329 (1998)
[Publications] Msanori Hisaoka: "Detection of TLS/FUS-CHOP fusion transcripts in myxoid/round cell liposarcomas by nested reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues." Diagnostic Molecular Pathology. 7.2. 96-101 (1998)
[Publications] Seiji Tsuji: "Peripheral primitive neuroectodermal tumour of the lung:report of two cases." Histopathology. 33.4. 369-374 (1998)
[Publications] Seiji Tsuji: "Detection of SYT-SSX fusion transcripts in synovial sarcoma by reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues." The American Journal of Pathology. 153.6. 1807-1812 (1998)
[Publications] Masanori Hisaoka: "Primary synovial sarcoma of the lung: report of two cases of the rare pulmonary sarcoma confirmed by molecular detection of SYT-SSX fusion gene transcripts." Histopathology. in press.
[Publications] Masanori Hisaoka: "Molecular detection of EWS-FLI1 chimeric transcripts in Ewing family tumors by nested reverse transcription-polymerase chain reaction: Application to archival paraffin-embedded tumor tissues." APMIS. in press.
[Publications] Masanori Hisaoka: "Retroperitoneal liposarcoma with combined well-differentiated and myxoid malignant fibrous histiocytoma-like myxoid areas." The American Journal of Surgical Pathology. in press.

1998 Fiscal Year Annual Research Report

ヒト軟部腫瘍における染色体および遺伝子異常の解析

Principal Investigator

橋本 洋 産業医科大学, 医学部, 教授 (10128069)

Research Products

[Publications] Masanori Hisaoka: "Diagnostic implication of Kaposi's sarcoma-associated herpesvirus with special reference to the distinction between spindle cell hemangioendothelioma and Kaposi's sarcoma." Archives of Pathology & Laboratory Medicine. 122.1. 72-76 (1998)

[Publications] 青木隆敏: "粘液状軟部腫瘍の鑑別診断:パラフィン包埋組織を用いたFISH法の応用" 病理と臨床. 16.2. 161-166 (1988)

[Publications] Masanori Hisaoka: "Intranodal palisaded myofibroblastoma with so-called amianthoid fibers: A report of two cases with a review of the literature." Pathology International. 48.4. 307-312 (1998)

[Publications] Yosuke Morimitsu: "Dermatofibrosarcoma protuberans and its fibrosarcomatous variant with areas of myoid differentiation:A report of three cases." Histopathology. 32.6. 547-551 (1998)

[Publications] Masanori Hisaoka: "Dermatofibrosarcoma protuberans with fibrosarcomatous areas: Molecular abnormalities of the p53 pathway in fibrosarcomatous transformation of dermatofibrosarcoma protuberans" Virchows Archiv. 433.4. 323-329 (1998)

[Publications] Msanori Hisaoka: "Detection of TLS/FUS-CHOP fusion transcripts in myxoid/round cell liposarcomas by nested reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues." Diagnostic Molecular Pathology. 7.2. 96-101 (1998)

[Publications] Seiji Tsuji: "Peripheral primitive neuroectodermal tumour of the lung:report of two cases." Histopathology. 33.4. 369-374 (1998)

[Publications] Seiji Tsuji: "Detection of SYT-SSX fusion transcripts in synovial sarcoma by reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues." The American Journal of Pathology. 153.6. 1807-1812 (1998)

[Publications] Masanori Hisaoka: "Primary synovial sarcoma of the lung: report of two cases of the rare pulmonary sarcoma confirmed by molecular detection of SYT-SSX fusion gene transcripts." Histopathology. in press.

[Publications] Masanori Hisaoka: "Molecular detection of EWS-FLI1 chimeric transcripts in Ewing family tumors by nested reverse transcription-polymerase chain reaction: Application to archival paraffin-embedded tumor tissues." APMIS. in press.

[Publications] Masanori Hisaoka: "Retroperitoneal liposarcoma with combined well-differentiated and myxoid malignant fibrous histiocytoma-like myxoid areas." The American Journal of Surgical Pathology. in press.

橋本洋産業医科大学, 医学部, 教授 (10128069)