1997 Fiscal Year Final Research Report Summary
Analysis of a novel gene RA3, expression of which is upregulated during cell proliferation
| Project/Area Number |
08670238
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Chiba University |
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Principal Investigator |
SHIRASAWA Hiroshi Microbiology I, Chiba University Professor, 医学部, 教授 (00216194)
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| Project Period (FY) |
1996 – 1997
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| Keywords | HPV / E6 / cell proliferation |
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| Research Abstract |
Cellular genes affected by human papillomavirus type 16 (HPV16) E6 protein were cloned by a differential hybridization technique. Two thirds of the cDNA clones were those that regulated during the cell cycle, such as HMG-IY (high mobility group protein-I[Y]), prothymosin-alpha, or ornithine decarboxylase. An unknown cDNA clone, RA3 was expressed during cell proliferation, and the expression was repressed by cell contact inhibition. Furthermore, repression of the RA3 expression was relieved by the expression of HPV16 E6.
The RA3 cDNA was 1.3kb in length, and have no homology to other known genes. No known motif was also found in this new gene.
The expression of the RA3 gene was correlated with the expression of the prothymosin-alpha and JUN.The expression of the prothymosin-alpha was activated by the HPV16E6 through the E2F motif of the c-myc gene. It was revealed that heterodimer complex of JUN and ATF2 is induced by the HPV16E6, followed by activation of genes that has CRE motif.
Taken together, the RA3 gene appears to be regulated by the c-myc or JUN/ATF2 and involved in the mechanisms of the cell proliferation.
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[Publications] Kinoshita, T., Shirasawa, H., Shino, Y., Shimizu, K., Moriya, H.and Simizu, B: "Human papillomavirus type 16 E6 protein up-regulates the expression of the high mobility group protein HMG-I (Y) gene in mouse 10T1/2 cells." Virus Res.42. 119-125 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Kinoshita, T., Shirasawa, H., Shino, Y., Moriya, H., Desbarats, L., Eilers, M.and Simizu, B.: "Transactivation of prothymosin-alpha and c-myc promoters by human papillomavirus type 16 E6 protein." Virology. 232. 53-61 (1997)
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「研究成果報告書概要(欧文)」より
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