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1997 Fiscal Year Final Research Report Summary

Identification of the molecular epitope associated with mesangical cell function.

Research Project

Project/Area Number 08670241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNiigata University

Principal Investigator

OITE Takashi  Niigata Univ.School of Medecine, Institute of Nephrology Dept.of Cellular Physiology, Professor and Director, 医学部, 教授 (60018744)

Co-Investigator(Kenkyū-buntansha) MORIOKA Tesuo  Niigata Univ.School of Medecine, Institute of Nephrology Dept.of Cellular Physio, 医学部, 助教授 (00210146)
Project Period (FY) 1996 – 1997
Keywordsrenal glomerulus / mesangial cell / vascular endothelial cell / extracellular matrix / cell adhesion molecules / cell-cell interction / regulation of cell function
Research Abstract

In the glomerulus, the endocapillary region is a closed space surrounded by the glomerular basement membrane comprised of a capillary lumen, endothelial cells (ECs), mesangical cells (MCs), and mesangical matrices. Our working hypothesis is that loss of physical and functional regulation by intrinsicECs and/or matrices may induce MCs dysfunction, leading to the pathological changes such as proliferation, enhanced extracellular matrix synthesis, MCs migration and mesangical interposition in the capillary region. By the aid of reseach grant (No.08670241,1996-1997) from the Ministry of Education, Science and Culture, we have got the results summarized up as the followings ;

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] T.Morioka et.al: "Anti-DNA antibody derived from an SLE patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo." Clin exp Immunol. 104. 92-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Oite et.al: "A specific Thy-1 molecular epitope expressed on rat mesangial cells." Exp Nephrol. 4. 350-360 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Narisawa-Saito et.al: "Thy-1-mediated phosphatidylinositol turnover in cultured rat glomerular mesangial cell." J Cell Physiol. 168. 705-710 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Narisawa-Saito et.al: "Thy-1 molecule associates with protein tyrosine kinase in rat mesangial cells." Clin exp Immunol. 106. 86-90 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 追手巍: "巻頭言 メサンギウム細胞" 腎と透析. 43. 169-170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Omori et.al: "Quantification of apoptotic cells using isolated glomeruli." Nephron. 77. 474-478 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Oite et.al: "The specific Thy-1 molecular epitope recognized by the monoclonal antibody, 1-22-3, is associated with points anchoring MCs and ECs in vitro and in vivo" Exp Nephrol. 4. 350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Narisawa-Saito et.al: "Incubaion of MCs with the two monoclonal antibodies against Thy-1.1, OX-7 and 1-22-3, resulted in increased inositol triphosphate levels and inclreased intracellular Ca++ concentration.In the above two assays, the rise levels by 1-22-3 were predominantly greater than those produced by OX-7" J Cell Physiol. 168. 705 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Narisawa-Saito et.al: "Thy-1 molecule transduces cell-signals associated with protein-tyrosine kinase such as p59fyn and p56/53lyn" Clin exp Immunol. 106. 86 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Oite et.al: "In order to confirm some specfic, functionnal epitopes of Thy-1.1, we have used COS cells transfected with cDNA for Thy-1.1.Now, we are going to determine the specific epitope detecting with a monoclonal antibody 1-22-3 using epioptemapping kit (manscript, in preparation)" Exp Nephrol. 4. 350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Omori et.al: "When we have examined the mechanisms of glomerular injury induced by anti-Thy-1.1 antibody, we developed a new method for quantifying apoptotic cells within glomerli." Nephron. 77. 33 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kobauyashi et.al: "Using two monoclonal antibodies, we have analyzed profiles of inflammmaroty cell infiltration in anti-Thy-1 models. As a result, there ware significant diffarence in nephritogenicity, inflammatory features and prognosis." Nephron. 77. 333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Oite et.al: "Using high-coltage electron microscopy (National Institute for Physiological Science, Okazaki), we were able to examine the broader areas in the semi-three dimensional phase of samples." Exp Nephrol. 4. 350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morioka T,et.al: "Anti-DNA antibody derived from an SLE patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo." Clin.exp.Immunol. 104. 92-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oite T,et.al: "A specific Thy-1 molecular epitope expressed on rat mesangial cells." Exp Nephrol. 4. 350-360 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Narisawa-Saito M,et.al: "Thy-1-mediated phospatidyl-inositol turnover in cultured rat glomerular mesangial cell." J.Cell.Physiol. 168. 705-710 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Narisawa-Saito M,et.al: "Thy-1 molecule associates with protein tyrosine kinase in rat mesagial cell." Clin.exp.Immunol. 106. 86-90 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Omori S,et.al.: "Quantification of apoptotic cells using isolated glomeruli." Nephron. 77. 474-478 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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