1998 Fiscal Year Final Research Report Summary
Intracellular signal transduction of Tcell activated with bacterial super-antigen
| Project/Area Number |
08670320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
FUJIMAKI Wakae Tokyo Women's Medical University School of Medicine, Lecture Instructor, 医学部, 講師 (90256496)
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| Co-Investigator(Kenkyū-buntansha) |
YAGI Junji Tokyo Women's Medical University School of Medicine, Lecture Instructor, 医学部, 講師 (70182300)
IMANISHI Ken'ichi Tokyo Women's Medical University School of Medicine, Associate Professor, 医学部, 助教授 (80124527)
UCHIYAMA Takehiko Tokyo Women's Medical University School of Medicine, Professor, 医学部, 教授 (00050550)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Bacterial super-antigen / T-cell / signal transduction |
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| Research Abstract |
Biological rensponses after administration of bacterial super-antigen (SAg) into mammal were studied. Two typical bacterial SAgs were used for the following experiments ; toxic shock syndrome toxin -1 (TSST-1) and enthelotoxin A-E (SEs) which were derived from staphylococcus. Immunotolerance to SAg can be induced in mice pretreated with SAg. In this state, IL-2 production from CD4^+ T cell were suppressed and this suppression was reversed after IL-2 replacement. in the serial study, we also found that T cell differentiation was modulated by retinoic acid.
The cause of neonatal TSS-like exanthematous disease (NTED) has not been known, but in the serial study, we found that TSST-1 play an important role in developing this disease. Although the course of the disease is rash, the prognosis is relatively favorable. Based on these findings, the difference in the T cell response according to their maturation status was studied. Induction of immunotolerance in human derived cells were studied in mature thymic cells, in T cells from cord blood and in peripheral T cells. Immunotolerance were more easily induced in thymic cells and in T cells from cord blood than peripheral T cells.
Study on signal transduction pathway in these cells revealed that thyrosine phosphorylation of CD3-zeta chain was remarkably different among these T cells from different origin. It is considered that the difference of the phophorylation level among these cells is the possible mechanism which regulates the T cell response since the CD3-zeta chain is one of the most upstream molecules. We are now examining the tyrosine kinases which might be involved in the CD3-zeta chain phosphorylation.
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[Publications] Imanishi, K., Seo, K., Kato, H., Miyoshi-Akiyama, T., Zhang, RH., Takanashi, Y., Imai, Y., and Uchiyama, T.: "Post-thymic maturation of migrating human thymic single-positive T cells ; Thymic CD1a^-CD4^+ T cells are more susceptable to anergy induction by toxic shock syndrome toxin-1 than cord blood CD4^+ T cells." J.Immunol.Vol.160. 112-119 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Takahashi, N., Nishida, H., Kato, H., Imanishi, K., Sakata, Y., and Uchiyama, T.: "Exanthematous disease induced by toxic shock syndrome toxin 1 in the early neonatal period" The Lancet.351. 1614-1619 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Kuroda, K., Yagi, J., Imanishi, K., Yan, XJ., LiXY., Fujimaki, W., Kato, H., Miyoshi-Akiyama, T., Kumazawa, Y., Abe H., and Uchiyama, T.: "Implantation of IL-2-Osmotic Pump Prolongs the Survival of Superantaigen-Reacative T Cells Expressed in Mice Injected with Bacterial Superantigen." The Journal of Immunology.Vol.157. 1422-1431 (1996)
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「研究成果報告書概要(欧文)」より
