1998 Fiscal Year Final Research Report Summary
Mechanism of preventive and suppressive effects by interferon on hepatocarcinogenesis
| Project/Area Number |
08670610
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka City University, Medical School |
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Principal Investigator |
KUROKI Tesuo Osaka City University, 3rd.Department of Internal Medicine, Professor, 医学部, 教授 (30047328)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIGUCHI Shuhei Osaka City University, 3rd.Department of Internal Medicine, Assistant Professor, 医学部, 講師 (10192246)
SEKI Shuichi Osaka City University, 3rd.Department of Internal Medicine, Associate Professor, 医学部, 助教授 (50145778)
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| Project Period (FY) |
1996 – 1998
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| Keywords | hepatocarcinogenesis / interferon / basic FGF / prevention / anti-proliferative effects / metastasis |
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| Research Abstract |
To elucidate how the down-regulation of basic fibroblast growth factor (bFGF) in hepatocellular carcinoma by interferon (IFN) - alpha and -beta is related to the antiproliferative effects of lFN- alpha and-beta and to determine whether bFGF and IFNs can alter the invasiveness of hepatocellular carcinoma, I examined four hepatoma cell lines (PLC/PRF/5, HLF, HepG2, Huh-7). Anti-proliferative effects of IFN-alpha and-bete were strongest for PLC/PRF/5, relatively weak for Huh-7, and not observed for HLF and HepG2. IFN- gamma did not effect on all cell lines. The expression of bFGF was intense in HLF, PLC/PRF/5, Huh-7, and HepG2 in that order. The down-regulation of bFGF by IFN- alpha and -beta was observed only for PLC/PRF/5, and bFGF affected cell proliferation in hepatoma cell line cultures. These findings suggest that down-regulation of bFGF by lFN-alpha and -beta may be one of the mechanisms responsible for the anti-proliferative effects of lFN-alpha and -beta. In my in vitro invasion system, IFNs inhibited the invasiveness of hepatoma cell lines slightly, and bFGF did not increase their invasiveness.
Conclusion : (1) IFN - alpha and -beta inhibited the proliferation of hepatoma cell lines partly by down-regulation of bFGF. ; (2) IFNs decreased the invasiveness of hepatoma cell lines slightiy. ; and (3) bFGF had no effect on the invasiveness of hepatoma cell lines.
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