1997 Fiscal Year Final Research Report Summary
Microwave coagulation for hepatocellular carcinoma.
| Project/Area Number |
08670617
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KAWAMOTO Chiaki Jichi Med.Sch.Medicine Lecturer, 医学部, 講師 (30169776)
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| Co-Investigator(Kenkyū-buntansha) |
HOZUMI Masanori Jichi Med.Sch.Medicine Assistant, 医学部, 助手
ISODA Norio Jichi Med.Sch.Medicine Assistant, 医学部, 助手 (30275680)
IDO Kenichi Jichi Med.Sch.Medicine Assistant Professor, 医学部, 助教授 (80112621)
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| Project Period (FY) |
1996 – 1997
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| Keywords | hepatocellular carcinoma / microwave coagulation |
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| Research Abstract |
Clinical study
(1) Evaluation of the therapeutic efficacy after LMC
Clinically, the dissapearance of enhanced effect on CT imaging (a low density area on enhanced CT after LMC that is larger than the tumor size) was useful..
(2) Histological examination after microwave irradiation
Around the area of coagulation necrosis, liver cells were aggregated, but nucleus was intact in H.E.stain. Histologically, these liver cells were intact in H.E.stain and electron microscope, but in future these cells became necrosis.
We examined cytochrome C oxidase. Before treatment, cytochrome C oxidase activity wa seen. After microwave irradiation, there was no cytochrome C oxidase activity in mitochondoria. But, the form of mitochondria, nucleus, and other cell-organs were intact.
Initially microwave effects was considered thermal effexts. But, from these results, there may be unknown mechanisms to the liver.
Experimental Results
(1) The results of microwave effects (60W,1min.) to the vessels.
Irradiation was performed near the portal vein and the hepatic vein (distance is 2mm).
Desquamation of epithelial cells and degeneration of collagen fibers occured. But, portal vein and hepatic vein were not occuluded. Further, bile duct and hepatic artery were intact.
It was condidered that large blood vessels does not sustain injury.
(2) Microwave effects on canine liver
The center of coagulated area which was inserted electrode became carbonized.
Around carbonized area, coagulation necrosis occured.
Around the area of coagulation necrosis, liver cells were aggregated, but nucleus was intact in H.E.stain. Histologically, these liver cells were intact, but in future these cells became necrosis. Around aggregated cells, sinusoidal dilatation and congestion were seen.
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