1997 Fiscal Year Final Research Report Summary
Identification of pulmonary sarcoidosis related antigen.
| Project/Area Number |
08670688
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kurume University School of Medicine |
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Principal Investigator |
OIZUMI Kotaro Kurume Univ., Sch.Med., Dep.Medicine, Professor, 医学部, 教授 (70006097)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHINO Tomoaki Kurume Univ., Sch.Med., Dep.Immunol., Instructor, 医学部, 助手 (00261066)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Pulmonary sarcoidosis / Antigen / T lymphocytes / BALF / T cell receptor |
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| Research Abstract |
Sarcoidosis is a systemic granulomatous disease of unknown etiology characterized by the pronounced accumulation of CD4+T cell and macrophages in the affected organs. TCR variable (V) alpha and Vbeta gene usage in patients with sarcoidosis is still a matter of discussion. In this investigation, we analyzed TCR-Valpha and-Vbeta gene usage in bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) of 30 patients with active pulmonary sarcoidosis using an adapter ligation method, reverse transcriptase-polymerase chain reaction (RT-PCR), and sequence-specific oligonucleotide probe (SSOP) analyzes. There was no significant difference in TCR-Valpha or-Vbeta gene usage between BALF (n=12) or PBMC (n=27) of patients and PBMC of healthy subjects (n=10). Neither selective TCR-Valpha nor-Vbeta expansion was observed in the paired BALF and PBMC from seven of nine patients. However, selective expansions were observed in a few TCR-Valpha or-Vbeta subsets in the BALF or PBMC of some individuals. Although a modest increase in a few TCR-Valpha or-Vbeta subsets was observed in the BALF or PBMC of some individuals, the increased TCR-Valpha or-Vbeta subsets were not closely associated with the HLA-DRB1, DQA1, DQB1, and DPB1 alleles of these patients. These results suggest that TCR-Valpha or-Vbeta gene usage is not restricted in both lung and peripheral blood in the majority of patients with active pulmonary sarcoidosis.
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Research Products
(12 results)
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[Publications] Hoshino, T., Ohta, A., Nakao, M., Ota, T., Inokuchi, T., Matsueda, S., Gouhara, R., Yamada, A., Itoh, K., Oizumi, K: "Increase of TCRgammadelta+ T cells in peripheral blood of three patients with adults still's deisease" J.Rheumatology. 23. 124-129 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shjchifo, S., Hoshino, T., Koufuji, K., Hayashi, A., Kawamoto, M., Kikuchi, M., Higuchi, T., Ichiki, M., Oizumi, K., Itoh, K.: "Detection of MAGE-4 protein in sera of lung cancer patients" Jpn. J.Cancer Res. 88. 414-419 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hoshino, T., Seki, N., Kikuchi, M., Kuramoto, T., Iwamoto, O., Kodama, I., Koufuji K., Takeda, J., Itoh, K.: "HLA-class I-resticted and tumor-specific CTL in tumor-infiltrating lymphocystes of patients with gastric cancer" Int. J.Cancer. 70. 631-638 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hoshino, T., Suzuki, R., Tsuruta, Y., Matsutani, T., Kikuchi, M., Kawamoto, M., Gouhare, R., Shiraishi, T., Mochizuki, M., Itoh, K., Oizumi, K.: "Non-restricted T cell receptor (TCR) -Valpha and -Vbeta gene usage in patients with pulmonary sarcoidosis." Clin.Exp. Immunol.108. 529-538 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohba, H., Yoyokawa, T., Yasuda, S., Hoshino, T., Itoh, K., Yamasaki, N.: "Spectroscopic analysis of the cytoagglutinating activity of abrin-b isolated from Abrus precatorius seeds against leukimic cells." Biosci. Biotechnol. Biochem.61. 737-739 (1997)
Description
「研究成果報告書概要(欧文)」より
