1996 Fiscal Year Annual Research Report
生理活性ペプチドによる血管のオートクリン-パラクリン型緊張・増殖制御機構の解明
| Project/Area Number |
08670801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Research Institution | Kyushu University |
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Principal Investigator |
西村 淳二 九州大学, 医学部, 助教授 (90237727)
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| Keywords | 生理活性ペプチド / 血管平滑筋 / オートクリン / 血管増殖 |
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| Research Abstract |
本研究の目的は生理活性ペプチドによるオートクリン作用について明かにすることである。まず、アドレノメジュリンが、どの様な細胞に発現し、どの様な細胞に作用を及ぼすかについて明らかにした。その結果、アドレノメジュリンが心臓、腸管、血管、膀胱、気道等のいろいろな細胞に発現していることが明らかとなった。しかしながら、その平滑筋に対する作用は、限られていることがわかった(Nishimura et al.,1997、研究発表参照)。また、ANP(Seguchi et al.,1996)、CGRP(Fukuizumi et al.,1996),bradykinin(Eguchi et al.,in press)等の生理活性ペプチドに対する血管作用を明らかにした。気道、肺血管および冠動脈におけるendothelinのオートクリン作用について現在投稿中である。また、血管増殖に対する作用に関して報告をおこなった(Jahan et al.,1996 ; miyagi et al.,BBRC,1996)。さらに、P_<2U>受容体(miyagi et al.,J.Physiol.1996 ; miyagi et al.,Br.J.Pharmacol.1996),calponin(Sakihara et al.,1996),rho kinase(Nishimura et al,1996 ; Niiro et al.,in press)について報告を行った。カルシウム情報系に関する報告も行った(Higuchi et.al.,1996 ; Sakihara et al.,1996 ; Chen et al,in press ; hiroshi et al.,in press)。
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[Publications] Jahan H: "Endothelin-1 and angiotensin II act as progression but not competence growth factors in vascular smooth muscle cells." Eur.J.Pharmacol.295. 261-269 (1996)
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[Publications] Seguchi H: "The mechanism of relaxation induced by atrial natriuretic peptide in the porcine renal artery." Br.J.Pharmacol.118. 343-351 (1996)
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[Publications] Miyagi Y: "Dual regulation of cerebrovascular tone by UTP : P_<2U> receptor-mediated contraction and endothelium-dependent relaxation" Br.J.Pharmacol.118. 847-856 (1996)
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[Publications] Sakihara C: "Expression of calponin mRNA in porcine aortic endothelial cells." Biochem Biophys Res Commun. 222. 195-200 (1996)
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[Publications] Miyagi Y: "P_<2U> purinergic activation leads to the cell cycle progression from the G_1 to the S and M phases but not from the G_0 to G_1 phase in vascular smooth muscle cells in primary culture." Biochem Biophys Res Commun. 222. 652-658 (1996)
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[Publications] Miyagi Y: "P_<2U> rcceptor is linked to cytosolic Ca^<2+> transient and release of vasorelaxing factor in bovine endothelial cells in situ." J Physiol. 492. 751-761 (1996)
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[Publications] Fukuizumi Y: "The effects of calcitonin gene-related peptide on the cytosolic calcium concentratin and force in the porcine coronary artery." J Pharmacol Exp Ther. 278. 220-231 (1996)
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[Publications] Higuchi Y: "CPA induces a sustained increase in [Ca^<2+>]i of endothelial cells in situ and relaxes porcine coronary artery." Am.J Physiol. 270. H2038-H2049 (1996)
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[Publications] Sakihara C: "The direct inhibitory effect of chlorpromazine on smooth muscle of the porcine pulmonary artery." Anesthesiology. 85. 616-625 (1996)
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[Publications] Nishimura J: "Expression of rhoA and rho kinase mRNAs in porcine vascular smooth muscle." Biochem Biophys Res Commun. 227. 750-754 (1996)
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[Publications] Nishimura J: "The relaxant effect of adrenomedullin on particular smooth muscles despite a general expression of its mRNA in smooth muscle,endothelial and epithelial cells." Br.J.Pharmacol.120. 193-200 (1997)
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[Publications] Niiro N: "Up-regulation of RHO A and RHO-kinase mRNAS in the rat myometrium during pregnancy." Biochem Biophys Res Commun. (in press). (1997)
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[Publications] Chen X: "Resting load modulates the myofilament Ca^<2+> -sensitivity in rabbit cerebral arteries." J Cereb Blood Flow Metab. (in press). (1997)
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[Publications] Hiroishi G: "The differential effects of diltiazen and nitroglycerin on cytosolic Ca^<2+> concentration and on force in the bovine ophthalmic artery." Invest Ophthalmol Vis Sci. (in press). (1997)
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[Publications] Eguchi D: "Mechanism of contraction induced by bradykinin in the rabbit saphenous vein." Br J Pharmacol. (in press). (1997)
