1997 Fiscal Year Final Research Report Summary
Induction of CD4 antigen in B lymphocytes by Epstein-Barr virus infection
| Project/Area Number |
08670844
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | HOKKAIDO UNIVERSITY |
|---|
Principal Investigator |
KIKUTA Hideki Hokkaido Univ.Sch.of Med.Ass.Pro., 医学部, 助教授 (30271654)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKANO Motohiko Hokkaido Univ.Sch.of Med.Ass., 医学部・附属病院, 助手 (50261300)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Keywords | EBV / CD4 / HHV-6 / HIV-1 / chemokine |
|---|
| Research Abstract |
1.Fifteen lymphoblastoid cell lines (LCLs) were established by infection of B95-8 Epstein-Barr virus (EBV) in human peripheral blood mononuclear cells. The frequencies of CD4 antigen-positive cells varied among 15 LCL_s(range 0% to 47%).
2.mRNA of CCR5 and CXCR4 was not expressed in 15 LCL_s.
3.One cell line which was expressed CD4 antigen in 90% cells was obtained by the magnetic beads.
4.HHV-6 could not infect 15 LCL_s, indicating that CD4 antigen is not receptor for HHV-6. HIV-1 could replicate in all 15 LCL_s regardless of frequencies of CD4 antigen-positive cells, Indicating that HIV-1 may infect LCL_s by using another receptor.
|
