1997 Fiscal Year Final Research Report Summary
Basic Research for Gene Therapy of Congenital Hyperbilirubinemia
| Project/Area Number |
08670878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SATO Hiroshi Shiga University of Medical Science, Medical School Biology Associate Professor, 医学部, 助教授 (90090430)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Congenital Hyperbilirubinemia / UGT1A1 / Bilirubin / UDP-Glycosyltransferase / Crigler-Najiar Syndrome / Gene Therapy |
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| Research Abstract |
There has been no sufficient data for the expression of bilirubin UDP-glycosyltransfearse gene (UGT1A). So, we fist tried to clarify the regulation of the UGT1A1 expression as a first step of gene therapy, and we analyzed the promoter region (up to-3190) of UGT1A1 by transient transfection assay to identify transcriptionally regulatory sequences and found two elements ; one was between-1362 and-1220 and the other between-113 and-70. The proximal one (PE) consisted of two elements, E-box (-104 to-95) and HNF-1 site (-91 to-79). Distal element had sequences similar to those of AP-1 and CREB,but we could not demonstrate the bindings of the factors to DE by gel mobility-shift assay.
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[Publications] Yamamoto K,Soeda Y,Kamisako T,Hosaka H,Fukano M,Sato H,Fujiyama Y,Adachi Y,Satoh Y,Doida Y,Bamba T: Mutation analysis of seven patients with Crigler-Najjar syndrome type II.J.Hum Genet. (in press).
Description
「研究成果報告書概要(欧文)」より
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