1997 Fiscal Year Final Research Report Summary
Telomerase Activity of the Blood Cells in Patients with Myeloproliferative Disorders in Childhood
| Project/Area Number |
08670891
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NISHIMURA Shinichiro Hiroshima University Medical Hospital Research Associate, 医学部・附属病院, 助手 (00228222)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Satoshi Hiroshima University School of Medicine Research Associate, 医学部, 助手 (20243610)
HIYAMA Eisou Hiroshima University Medical Hospital Assistant Professor, 医学部・附属病院, 講師 (00218744)
UEDA Kazuhiro Hiroshima University School of Medicine Professor, 医学部, 教授 (30112189)
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| Project Period (FY) |
1996 – 1997
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| Keywords | telomere / telomerase / Myeloproliferative Disorders in Childhood |
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| Research Abstract |
It is not known exactly whether myeloproliferative disorders ; MD in childhood (juvenile chronic myelogenous leukemia ; JCML,transient myeloproliferative disorder ; TMD) are ture malignacy or not. To address this question, we examined telomerase expression of the blood cells in patients with MD.Telomerase activity was found in all leukemic cells of 10 patients with acute lymphoblastic leukemia ; ALL.Terminal restriction fragment ; TRF lengths in the leukemic cells were shorter than that in normal blood cells, although it was not significant. In two patients with JCML and one with TMD,telomerase activity was also found, but TRF lengths in the blood cells were within 8kb to 15kb consist with those of blood cells from age-matched normal donors. Because normal lymphocytes and hematopoietic progenitor cell express telomerase activity, further investigation is needed to clarify the relationship between hematological malignacy and telomerase activiry.
In the next experiment, we examined the proportion of delected mitochondrial DNA in blood cells from three patients with Peason syndrome. Southern blot analysis represented that the proportion of deleted mitochondorial DNA in peripheral blood and bone marrow has a tendency to correlate to the severity of hematological manifestation.
We will study the relationship between mitochondorial abnormality and telomerase acticity in blood cells from patients with MD in future.
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